Anti-Mouse IL-18BP
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Goat Immunogen Purified Recombinant IL-18BP (>98%) Endotoxin Level <10 EU/ µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830652 Applications and Recommended Usage? Quality Tested by Leinco Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as PN:G505. The detection sensitivity of this indirect ELISA for Mouse IL-18BP is approximately 0.3 ng/well. Western Blotting: To detect Mouse IL-18BP this polyclonal antibody can be used at a concentration of 0.5 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IL-18BP is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Mouse IL-18BP bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse IL-18BP (at 1 ng/ml) is 10 - 30 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Anti-Mouse Interleukin-18 Binding Protein (IL-18BP) recognizes Mouse IL-18BP. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Mouse IL-18BP is a circulating glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 with high affinity and neutralizing its biological activity. IL-18BP has no amino acid sequence homology to IL-18 or IL-1 receptors. The gene for human IL-18BP encodes at least four isoforms by alternative splicing. The IL-18BP isoforms A and C each contain one immunoglobulin (Ig)-like C2-type domain while isoforms B and D lack the complete Ig domain. The complete Ig domain has been shown to be essential for IL-18BPs binding and neutralizing properties. Several poxviruses have been shown to encode proteins with sequence similarity to human and mouse IL-18BPs that also bind and inhibit IL-18 bioactivities. This could lead to viral modulation of host immune responses. Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org Technical Protocols N  |
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