Anti-Mouse Lefty-1
Anti-Mouse Lefty-1
Product No.: L220
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Clone 146903 Target Lefty-1 Formats AvailableView All Product Type Monoclonal Antibody Isotype IgG2a Applications ELISA Indirect , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen NS0-Derived Recombinant Mouse Lefty-1 Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831150 Applications and Recommended Usage? Quality Tested by Leinco Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as PN:R380. The detection sensitivity of this indirect ELISA for Mouse Lefty-1 is approximately 3 ng/well. Western Blotting: To detect Mouse Lefty-1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse Lefty-1 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rat Anti-Mouse Lefty-1 (Clone 146903) recognizes an epitope on Mouse Lefty-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org Technical Protocols  |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
