Anti-Mouse TSLP R
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Goat Immunogen NS0 - Derived Recombinant Mouse TSLP Receptor Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831966 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing TSLP R. A suggested method would be to stain cells expressing TSLP R with 0.25 µg per 1 - 2.5 x 106 cells in a total staining volume of ≤200 µl followed by PN:G641. Western Blotting: To detect Mouse TSLP R this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse TSLP R is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Mouse TSLP R bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse TSLP R (at 7.5 ng/ml) is 0.2 - 0.6 µg/ml. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Anti-Mouse Thymic Stromal-Derived Lymphopoietin Receptor (TSLP R) recognizes Mouse TSLP R. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Thymic stromal lymphopoietin receptor (TSLP R) is a member of the hematopoietin receptor family.1 TSLPR forms a heterodimeric complex with the IL-7 receptor alpha chain to form the receptor for thymic stromal derived lymphopoietin (TLSP), a cytokine involved in B- and T-cell function.2 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Sims, JE. et al. (2000) J Exp Med. 192:659 2. Pandey, A. et al. (2002) Gene. 284:161 Technical ProtocolsCyTOF®  N  |
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