Anti-Rat Agrin

Product No.: A385


Target Agrin Product Type Polyclonal Antibody Applications ELISA Cap , IHC , N , WB


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Antibody Details

Product Details

Reactive Species

Rat

Host Species

Goat

Immunogen

Purified Recombinant Rat Agrin (>98%)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

State of Matter

Lyophilized

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2828133

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.2-0.8 µg/mL. A suitable detection antibody is PN:A399 at a concentration of approximately 200 ng/ml. A suggested standard for this assay is PN:A344.
Western Blotting: To detect Rat Agrin this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Rat Agrin is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:A344.

Additional Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Rat Agrin bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Rat Agrin (at 0.016 ng/ml) is 0.001 - 0.004 µg/ml.
Immunohistochemistry: Suitable for use at concentration of 5-15 µg/mL.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Rat Agrin recognizes Rat Agrin. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.