Anti-SARS-CoV-2, Nucleocapsid (N) (COV19-5164)

Formulated in 0.01 M phosphate buffered saline, pH 7.2 and contains 0.1% sodium azide. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Liquid

This monoclonal antibody is purified by protein A chromatography or sequential differential precipitations.

This purified antibody is stable when stored at 2-8°C. Do not freeze.

Research Use Only

2-8°C Wet Ice

Anti-SARS-CoV-2 (Clone COV19-5164) is specific for the nucleocapsid of SARS-CoV-2 including variants: UK B.1.1.7, SA B.1.351, Brazil P.1, B.1.617.2 (Delta), B.1.1.529 (Omicron) & BA.4. It is non-reactive with Influenza A, Influenza B, Adenovirus & MERS but does cross-react with SARS.

The nucleocapsid protein is expressed in the internal nucleocapsid of SARS-CoV-2.

Best ELISA pair (capture/conjugate), COV19-5164/COV19-6429.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), is an enveloped, single-stranded, positive-sense RNA virus that belongs to the Coronaviridae family ¹. The SARS-CoV-2 genome, which shares 79.6% identity with SARS-CoV, encodes four essential structural proteins: the spike (S), envelope (E), membrane (M), and nucleocapsid protein (N) ². The N protein is 46 kDa and consists of two highly conserved structural domains, the N-terminal domain (NTD) and C-terminal domain (CTD), connected by a linker region. The NTD and CTD are involved in RNA binding and self-oligomerization, respectively ^{3, 4}. The primary function of the N protein is to bind to and package the viral RNA genome into a helical ribonucleoprotein complex ⁵. The N protein is also involved in other critical steps of the viral life cycle, including transcription, replication, and modulating infected cell signaling pathways ^{6, 7}. The N protein is abundantly expressed during infection and is highly conserved, sharing 90% amino acid homology with the SARS-CoV N protein ⁸. It is also immunogenic, and antibodies ^8,9 and memory T cells ^{10, 11} targeting the N protein are present in the sera of convalescent COVID-19 patients, identifying the N protein as a suitable candidate for vaccine development and diagnostic assays. Diagnostic assays based on the N protein effectively detect antibodies in the sera of patients infected with SARS-CoV-2 ¹². The N protein also contributes to immune evasion by antagonizing antiviral RNAi ¹³, suggesting its potential value as a targeted therapeutic.

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13. Mu J, Xu J, Zhang L, et al. Sci China Life Sci. 1-4. 2020.
14. Gorshkov, Kirill, et al. "SARS-CoV-2 Nucleocapsid Protein TR-FRET Assay Amenable to High Throughput Screening." ACS pharmacology & translational science 5.1 (2022): 8-19. Link
15. Park, Jun-Gyu, et al. "Animal Models of COVID-19: Transgenic Mouse Model." SARS-CoV-2. Humana, New York, NY, 2022. 259-289. Link