Recombinant Human CCL4L1

CCL4L1/platelet factor-4 variant (PF-4var), is a highly angiostatic chemokine¹ and a potent regulator of endothelial cell biology, which affects angiogenesis and vascular diseases.² CXCL4 is a member of the CXC chemokine family, which is mainly produced by platelets and known for its pleiotropic biological functions. CXCL4L1 exhibits a distinct subcellular localization has a distinct roles in inflammatory or homeostatic processes.³

The biological activity of Human CCL4L1 was determined by its ability to chemoattract human CCR5 transfected BaF3 mouse proB cells. The expected ED₅₀= 0.5-2.5 ng/ml.

NP_001001435

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Ala24

Lyophilized

The predicted molecular weight of Recombinant Human CCL4L1 is Mr 7.8 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 12 kDa.

7.8

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Struyf, S. et al.(2007) J. Leukos Biol. 82: 1519
2. Strieter, RM. et al.(2004) Circ Res. 95(9):855-7.
3. Romagnani, P. et al.(2007) Blood 109: 4127