Recombinant Human Erythropoietin Receptor

The erythropoietin receptor is a transmembrane protein belonging to the member of the cytokine receptor family. The receptor is tyrosine phosphorylated upon binding by erythropoietin and associates with and activates the tyrosine kinase, JAK2, which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. EPO-R transmits signals important for both proliferation and differentiation along the erythroid lineage.¹ Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis.

The biological activity of Human Epo R was determined by its ability to inhibit Epodependent proliferation of TF1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The expected ED₅₀ is 1 - 4 ng/ml in the presence of 0.2 U/ml of rhEpo.

Fc Fusion Protein

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Lyophilized

The predicted molecular weight of Recombinant Human Epo R is Mr 51.4 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 55-60 kDa.

51.4

This recombinant protein was 0.2 µm filtered and lyophilized from PBS with 0.1 mM EDTA and 0.05 % CHAPS, pH 7.2.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Siren, AL. et al. (2001) Proc Natl Acad Sci USA 98: 4044
2. Haroon, ZA. et al. (2003) Am J Pathol 163: 993