Recombinant Human FGF-18
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BackgroundFGF-18 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. It has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. Studies of the similar proteins in mouse and chick suggested that this protein is a pleiotropic growth factor that stimulates proliferation in a number of tissues, most notably the liver and small intestine. Knockout studies of the similar gene in mice implied the role of this protein in regulating proliferation and differentiation of midline cerebellar structures. Protein DetailsPurity >95% by SDS Page and HPLC Endotoxin Level <1.0 EU/µg as determined by the LAL method Biological Activity The biological activity of Human FGF-18 was determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors. The expected ED<sub>50</sub> is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 10<sup>6</sup> units/mg. Protein Accession No. Amino Acid Sequence AEENVDFRIH VENQTRARDD VSRKQLRLYQ LYSRTSGKHI QVLGRRISAR GEDGDKYAQL LVETDTFGSQ VRIKGKETEF YLCMNRKGKL VGKPDGTSKE CVFIEKVLEN NYTALMSAKY SGWYVGFTKK GRPRKGPKTR ENQQDVHFMK RYPKGQPELQ KPFKYTTVTK RSR State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Human FGF-18 is Mr 20.1 kDa. Predicted Molecular Mass 20.1 Storage and Stability The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day Ambient NCBI Gene Bank IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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