Recombinant Human FGF-18
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BackgroundFGF-18 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. It has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. Studies of the similar proteins in mouse and chick suggested that this protein is a pleiotropic growth factor that stimulates proliferation in a number of tissues, most notably the liver and small intestine. Knockout studies of the similar gene in mice implied the role of this protein in regulating proliferation and differentiation of midline cerebellar structures. Protein DetailsPurity >95% by SDS Page and HPLC Endotoxin Level <1.0 EU/µg as determined by the LAL method Biological Activity The biological activity of Human FGF-18 was determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors. The expected ED<sub>50</sub> is ≤ 4 - 24 ng/ml, corresponding to a specific activity of ≥ 2 x 10<sup>6</sup> units/mg. Protein Accession No. Amino Acid Sequence een vdfrihvenq trarddvsrk qlrlyqlysr tsgkhiqvlg rrisargedg dkyaqllvet dtfgsqvrik gketefylcm nrkgklvgkp dgtskecvfi ekvlennyta lmsakysgwy vgftkkgrpr kgpktrenqq dvhfmkrypk gqpelqkpfk yttvtkrsrr irpthpa N-terminal Sequence Analysis Met State of Matter Lyophilized SDS-Page Molecular Weight 19-24 kDa, reducing conditions Predicted Molecular Mass The predicted molecular weight of Recombinant Human FGF-18 is Mr 21 kDa. Formulation This rProtein was lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and Trehalose. NOTE: This differs from many of our other proteins for stability purposes. Reconstitution Reconstitute at 500 μg/mL in water. Storage and Stability The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day Ambient NCBI Gene Bank Certificate of AnalysisIMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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