Recombinant Human FGF-8a

Fibroblast growth factor-8 (FGF-8), also known as AIGF and HBGF, is a heparin binding growth factor belonging to the FGF family (1). Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation (2). Alternate splicing of FGF-8 mRNA creates eight secreted isoforms (a-h) in mice and four (a, b, e and f) in humans (3). FGF-8a expands the midbrain in transgenic mice, while FGF-8b transforms the midbrain into cerebellum. FGF-8 activates the “c” splice forms of receptors FGF R2, FGF R3 and FGF R4, with differential activity among the FGF-8 isoforms. Overexpression of FGF-8 has been shown to increase tumor growth and angiogenesis. FGF-8b shows the strongest receptor affinity and oncogenic transforming capacity, although isoforms a and e have been found in human tumors (4). The adult expression of FGF-8 is restricted to testes and ovaries.

The biological activity of Human FGF-8a was determined by a cell proliferation assay using NR6R-3T3 mouse fibroblasts. The expected ED₅₀=0.4 - 2µg/ml in the presence of 10µg/ml heparin.

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Lyophilized

The predicted molecular weight of Recombinant Human FGF-8a is Mr 21 kDa.

21

This recombinant protein was lyophilized from a 0.2 μm filtered solution in MOPS, EDTA, Dithiothreitol (DTT), and sodium sulphate (Na₂SO₄).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Flow Cytometry: PN:A106
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCR5. A suggested method would be to stain cells expressing CXCR5 with approximately 10 µl per test. A typical test sample constitutes approximately 50 µl of packed whole blood or 1 x 10⁵ continuous passage or activated cell cultures that have been centrifuged at 500 X g for five minutes. Labeling of the cells with the biotin conjugate should be followed by PN:A104, resuspended in 200-400 µl of 1X PBS.

1. Gemel, J. et al. (1996) Genomics 35:253
2. Ruess, B. et al. (2003) Cell Tissue Res. 313:139
3. Tanaka, S. et al. (2001) Digest. Dis. Sci. 46:1016
4. Olsen, SK. et al. (2006) Genes Dev. 20:185