Recombinant Human Galectin-3BP
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Protein DetailsPurity >95% by SDS Page and analyzed by silver stain. Endotoxin Level <0.1 EU/µg as determined by the LAL method Protein Accession No. Amino Acid Sequence vndgdmrlad ggatnqgrve ifyrgqwgtv cdnlwdltda svvcralgfe natqalgraa fgqgsgpiml devqctgtea sladckslgw lksncrherd agvvctnetr sthtldlsre lsealgqifd sqrgcdlsis vnvqgedalg fcghtvilta nleaqalwke pgsnvtmsvd aecvpmvrdl lryfysrrid itlssvkcfh klasaygarq lqgycaslfa illpqdpsfq mpldlyayav atgdallekl clqflawnfe altqaeawps vptdllqlll prsdlavpse lallkavdtw swgerashee veglvekirf pmmlpeelfe lqfnlslyws healfqkktl qalefhtvpf qllarykgln ltedtykpri ytsptwsafv tdsswsarks qlvyqsrrgp lvkyssdyfq apsdyryypy qsfqtpqhps flfqdkrvsw slvylptiqs cwnygfscss delpvlgltk sggsdrtiay enkalmlceg lfvadvtdfe gwkaaipsal dtnsskstss fpcpaghfng frtvirpfyl tnssgvdyyp yqsfqtpqhp sflfqdkrvs wslvylptiq scwnygfscs sdelpvlglt ksggsdrtia yenkalmlce glfvadvtdf egwkaaipsa ldtnssksts sfpcpaghfn gfrtvirpfy ltnssgvdhh hhhhhhhh N-terminal Sequence Analysis Val19 & Tyr455 State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Human M2BP, MAC-2BP, Galectin-3BP is 65 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 26-30 kDa, 60-69 kDa and 81-94 kDa (reducing conditions). Predicted Molecular Mass 65 kDa Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT with Trehalose. Reconstitution Reconstitute in PBS to 500 μg/mL Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day Ambient NCBI Gene Bank Applications and Recommended Usage ? (Quality Tested by Leinco) ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml. Flow Cytometry: PN:A106 Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCR5. A suggested method would be to stain cells expressing CXCR5 with approximately 10 µl per test. A typical test sample constitutes approximately 50 µl of packed whole blood or 1 x 105 continuous passage or activated cell cultures that have been centrifuged at 500 X g for five minutes. Labeling of the cells with the biotin conjugate should be followed by PN:A104, resuspended in 200-400 µl of 1X PBS. Technical ProtocolsIMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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