Recombinant Human IL-1 RI

Interleukin-1 receptor, type I (IL1R1), also known as CD121a, is a transmembrane glycoprotein and member of the IL-1 receptor family. As important mediators involved in many immune and inflammatory responses, the agonists IL-1α and IL-1β bind to IL1R1, leading to the signal transduction and activation of NF-κ B. IL1R1 also forms a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2 which is indispensible for the signaling processes (1). IL1R1 has been shown to interact with PIK3R1, Myd88 and IL1RAP (2-4). It is expressed predominantly by T cells, fibroblasts and endothelial cells.

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Asp21

Lyophilized

The predicted molecular weight of Recombinant Human IL-1 RI is Mr 36 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 55 kDa.

36

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Burns, K. et al. (2006) Curr. Biol. 16:2265
2. Reddy, SA. et al. (1995) J. Biol. Chem. 272:29167
3. Burns, K. et al. (2000) Nat. Cell Biol. 2:346
4. Huang, J. et al. (1997) Proc. Natl. Acad. Sci. (USA) 94:12829