Recombinant Human PDGF-AB

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Pricing & Details

Product. No.P128
Alternate Names
Glioma-Derived Growth Factor [GDGF], Osteosarcoma-Derived Growth Factor [ODGF]
Expression Host
E. coli
Species
Human
Prod No.
Size
Price
Avail.
Qty
Add to cart
P128-10 µg
10 µg
$279.00
In stock
Max:
Min: 1
Step: 1
P128-50 µg
50 µg
$839.00
In stock
Max:
Min: 1
Step: 1

Background

Platelet-derived growth factor AB (PDGF-AB) is a heterodimeric1 non-glycosylated polypeptide isomer of PDGF.2 The biological effects of PDGF are mediated by cell surface alpha and beta PDGF receptors, which, as a result of ligand binding, undergo dimerization.3 PDGF-AB interaction with the alpha PDGF receptor induces conformational changes in the ligand or receptor that facilitates efficient recruitment of beta PDGFR by this PDGF isoform.4 However, PDGF-AA is not likely to be physiologically significant activators of cells unless the cells express PDGFR alpha.5 PDGF-AB may promote wound healing by stimulating extracellular matrix synthesis, e.g. collagen. It may also play a role in scar formation through collagen deposition.6

Protein Details

Purity
>97% by SDS Page and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human PDGF-AB was determined by its ability to stimulate proliferation of NR6R-3T3 fibroblasts (Raines, E.W. et al., 1985, Methods Enzym. 109:749). The expected ED50 for this effect is typically 1.0 - 3.0 ng/mL when measured by 3H-thymidine incorporation.
Expression Host
E. coli
Protein Accession No.
P04085.1 (A chain) & P01127.1 (B chain)
Amino Acid Sequence
(Human PDGF-A) sieeavpavc ktrtviyeip rsqvdptsan fliwppcvev krctgccnts svkcqpsrvh hrsvkvakve yvrkkpklke vqvrleehle cacattslnp dyreedtgrp resgkkrkrk rlkpt, (Human PDGF-B) slgsltiaep amiaecktrt evfeisrrli drtnanflvw ppcvevqrcs gccnnrnvqc rptqvqlrpv qvrkieivrk kpifkkatvt ledhlackce tvaaarpvt
N-terminal Sequence Analysis
Ser87 (A chain) & Ser82 (B chain)
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human PDGF-AB is 14.3 kDa (A chain) & 12.3 kDa (B chain). However, the actual molecular weight as observed by migration on SDS-PAGE is 17.5 kDa (A chain) & 12.5 kDa (B chain) under reducing conditions.
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Helden, CH. et al. (1988) J Biol Chem. 263: 16493
2. Canalis, E. et al. (1994) Endocrinology 134: 2541
3. Giese, NA. et al. (1993) J Biol Chem. 268: 3625
4. Aaronson, SA. et al. (1991) J Biol Chem. 266: 20232
5. Bowen-Pope, DF. et al. (1993) J Biol Chem. 268: 4473
6. Xu, M. et al. (2000) Chinese Journal of Plastic Surgery and Burns 16: 78
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Prod No.
Description
P128
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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