Recombinant Human SDF-1α
Recombinant Human SDF-1α
Product No.: S111
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Alternate Names Stromal Cell-Derived Factor-1 Alpha, TLSF-a, CXCL12a, Pre-B Cell Growth Stimulating Factor (PBSF) Product Type Recombinant Protein Expression Host E. coli Cells Species Human/Rhesus Macaque/Feline |
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BackgroundSDF-1alpha (stromal cell-derived factor-1 alpha) is an alpha-chemokine for haematopoietic stem cells (HSC).4 SDF-1 alpha plays a role in two important processes of granule cell maturation - proliferation and migration - assisting in the achievement of appropriate cell number and position in the cerebellar cortex.5 SDF-1 alpha is also involved in the trafficking of hematopoietic progenitors and stem cells.6 It is expressed by liver cells and in liver diseases.7 Protein DetailsPurity ≥95% by SDS Page Endotoxin Level < 1.0 EU/mg as determined by the LAL method Biological Activity The biological activity of Human SDF-1α was determined by its ability to chemoattract 5 - 10 day cultured human T-lymphocytes, or chemoattract BaF/3 hCXCR4 cells. The expected ED<sub>50</sub> for these effects are typically 3 - 9 ng/ml, or 0.15 - 0.6 ng/ml, respectively. Protein Accession No. Amino Acid Sequence kpvslsyrc pcrffeshva ranvkhlkil ntpncalqiv arlknnnrqv cidpklkwiq eylekalnk N-terminal Sequence Analysis Lys22 State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Human SDF-1α is Mr 8.0 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 7 kDa (reducing conditions). Predicted Molecular Mass 8.0 Formulation This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA). Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day Ambient NCBI Gene Bank References & Citations1. Peiper, SC. et al. (2006) Methods Mol Biol. 332:115-27. Article Link 2. Peiper, SC. et al. (2005) Cancer Res.65(22):10450-6. Article Link 3. Peiper SC. et al. (2002) J Biol Chem. 277(27):24515-21. Article Link 4. Pei, G. et al. (2002) J Biol Chem. 277(51):49212-9. Article Link 5. Ratajczak MZ. et al. (2003) Folia Histochem Cytobiol. 41: 13 6. Luster AD et al. (2001) Development 128: 1971 7. Vannier JP et al. (2002) Eur Cytokine Netw 12: 223 8. James Dickenson et al. (2008) Iran J Allergy Asthma Immunol. 7: 61 IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
