Recombinant Human SDF-1α

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Pricing & Details

Product. No.S111
Alternate Names
Stromal Cell-Derived Factor-1 Alpha, TLSF-a, CXCL12a, Pre-B Cell Growth Stimulating Factor (PBSF)
Expression Host
E. coli
Species
Human/Rhesus Macaque/Feline
Prod No.
Size
Price
Avail.
Qty
Add to cart
S111-10 µg
10 µg
$249.00
In stock
Max:
Min: 1
Step: 1
S111-50 µg
50 µg
$739.00
In stock
Max:
Min: 1
Step: 1

Data

Background

SDF-1alpha (stromal cell-derived factor-1 alpha) is an alpha-chemokine for haematopoietic stem cells (HSC).4 SDF-1 alpha plays a role in two important processes of granule cell maturation - proliferation and migration - assisting in the achievement of appropriate cell number and position in the cerebellar cortex.5 SDF-1 alpha is also involved in the trafficking of hematopoietic progenitors and stem cells.6 It is expressed by liver cells and in liver diseases.7

Protein Details

Purity
≥95% by SDS Page
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Biological Activity
The biological activity of Human SDF-1α was determined by its ability to chemoattract 5 - 10 day cultured human T-lymphocytes, or chemoattract BaF/3 hCXCR4 cells. The expected ED50 for these effects are typically 3 - 9 ng/ml, or 0.15 - 0.6 ng/ml, respectively.
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
kpvslsyrc pcrffeshva ranvkhlkil ntpncalqiv arlknnnrqv cidpklkwiq eylekalnk
N-terminal Sequence Analysis
Lys22
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human SDF-1α is Mr 8.0 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 7 kDa (reducing conditions).
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Peiper, SC. et al. (2006) Methods Mol Biol. 332:115-27. Article Link
2. Peiper, SC. et al. (2005) Cancer Res.65(22):10450-6. Article Link
3. Peiper SC. et al. (2002) J Biol Chem. 277(27):24515-21. Article Link
4. Pei, G. et al. (2002) J Biol Chem. 277(51):49212-9. Article Link
5. Ratajczak MZ. et al. (2003) Folia Histochem Cytobiol. 41: 13
6. Luster AD et al. (2001) Development 128: 1971
7. Vannier JP et al. (2002) Eur Cytokine Netw 12: 223
8. James Dickenson et al. (2008) Iran J Allergy Asthma Immunol. 7: 61
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Prod No.
Description
S111
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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