Recombinant Mouse TNF-α

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Pricing & Details

Product. No.T156
Alternate Names
TNF-alpha, TNFSF2, Cachectin, Differentiation-Inducing Factor (DIF), Necrosin, Cytotoxin
Expression Host
E. coli
Species
Mouse
Prod No.
Size
Price
Avail.
Qty
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T156-10 µg
10 µg
$209.00
In stock
Max:
Min: 1
Step: 1
T156-25 μg
25 µg
$319.00
In stock
Max:
Min: 1
Step: 1
T156-50 µg
50 µg
$659.00
In stock
Max:
Min: 1
Step: 1

Background

TNF-α is a 17.5 kD protein that mediates inflammation and immunity caused by the invasion of viruses, bacteria, and parasites by initiating a cascade of cytokines that increase vascular permeability, thus bringing macrophages and neutrophils to the site of infection. TNF-α secreted by the macrophage causes the blood to clot which provides containment of the infection. TNF-α binding to surface receptors brings about various biologic activities that include cytolysis and cytostasis of many tumor cell lines In vitro, hemorraghic necrosis of tumors In vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse TNF-α was determined by by TNF mediated cytotoxicity in the mouse L-929 cell line, in the presence of the metabolic inhibitor actinomycin D (Matthews, N. et al., 1987. in Lymphokines and Interferons, a practical approach. M.J. Clemens, A.G. Morris and A.J.H Gearing, eds. IRL Press. p. 221). The expected ED50 for this effect is typically 0.02 - 0.05 ng/ml.
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
(ml rss)sqnssdk pvahvvanhq veeqlewlsq ranallangm dlkdnqlvvp adglylvysq vlfkgqgcpd yvllthtvsr faisyqekvn llsavkspcp kdtpegaelk pwyepiylgg vfqlekgdql saevnlpkyl dfaesgqvyf gvial
N-terminal Sequence Analysis
Met & Ser84
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse TNF-α is Mr 17 kDa. A small amount of recombinant protein lacking the N-terminal methionine and four additional amino acid residues is also present in the preparation.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. El-Harith, EHA. et al. (2004) Saudi Med J. 25: 135
2. Adolf, GR. et al. (1990) Infec Immun. 58: 3996
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.