Anti-Human TNF-α (Clone 1825)

Pricing & Details

Product No.T427
Clone
1825
Protein
TNF-α
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
TNF-alpha, TNFSF2, Cachectin, Differentiation-Inducing Factor (DIF), Necrosin, Cytotoxin
Isotype
Mouse
IgG1
Applications
N
Prod No.
Size
Price
Avail.
Qty
Add to cart
T427-25 µg
25 µg
$195.00
In stock
Max:
Min: 1
Step: 1
T427-100 µg
100 µg
$440.00
In stock
Max:
Min: 1
Step: 1
T427-500 µg
500 µg
$935.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human TNF-α (Accession # P01375)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human TNF-α bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human TNF-α (at 0.25 ng/ml) is 0.02 - 0.04 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 1825 recognizes an epitope on human TNF-α.
Antigen Distribution
TNF-α is secreted by macrophages, monocytes, neutrophils, T cells, B cells, NK cells, LAK cells.
Background
TNF-α is a 17.5 kD protein that mediates inflammation and immunity caused by the invasion of viruses, bacteria, and parasites by initiating a cascade of cytokines that increase vascular permeability, thus bringing macrophages and neutrophils to the site of infection. TNF-α secreted by the macrophage causes the blood to clot which provides containment of the infection. TNF-α binding to surface receptors brings about various biologic activities that include cytolysis and cytostasis of many tumor cell lines In vitro, hemorraghic necrosis of tumors In vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

Antigen Details

Protein
PubMed

References & Citations

1. El-Harith, EHA. et al. (2004) Saudi Med J. 25: 135
2. Adolf, GR. et al. (1990) Infec Immun. 58: 3996

Formats Available

Prod No.
Description
T427
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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