Anti-Human IRF3 (CT)

PN:I-518

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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Rabbit Anti-Human Interferon Regulatory Factor-3 (IRF3) recognizes an epitope near the C-terminus of Human and Mouse IRF3. This polyclonal antibody was purified using affinity chromatography.

Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms (1 for review). During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-α/β genes. IRF3 is present in an inactive form in the cytoplasm of most cells.² Following viral infection, IRF3 can be activated by IκB kinase-ε and TANK-binding kinase 1 (TBK1),^3,4 whereupon IRF3 translocates to the nucleus. IRF3 can also be activated by stimulation of toll-like receptor 3 (TLR3) by dsRNA.³ IRF3 exists as at least two distinct isoforms.

1. Malmgaard, L. (2004) J. Interferon & Cyto. Res. 24:439 2. Au, W. C. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11657 3. Fitzgerald, K. A. et al. (2003) Nat. Immunol. 4:491 4. Sharma, S. et al. (2003) Science 300:1148