Anti-Mouse/Human KLRG-1 – Biotin

IL-2-activated NK cells (A-LAK cells) from B6 (H-2b) mice

This Biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Liquid

This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

2F1 activity is directed against mouse and human KLRG1.

KLRG1 is expressed by subsets of natural killer cells and a small fraction of T cells but not by other lymphohematopoietic cells in both mouse and human.

Killer cell lectin-like receptor G1 (KLRG1) is a transmembrane glycoprotein with an extracellular C-type lectin-like domain and a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM)¹. KLRG1 plays an inhibitory role in human NK and T cells² and is frequently used as a marker of cellular differentiation, with memory cells and highly differentiated end-stage cells having a significantly higher percentage of expression than differentiating cells². Additionally, the proportion of CD8⁺ T cells expressing KLRG1 is age-related, increasing from ~ 40% of the CD8⁺ T cell pool in young adults to 90% in individuals over 65. KLRG1 is known to bind E-, N-, and R-cadherin^{1, 2}. Cadherin interactions are generally involved in cell-to-cell adhesion and additionally, KLRG1/E-cadherin interactions are involved in immune control².

2F1 was generated by immunizing Syrian hamsters with IL-2 activated NK (A-LAK) cells from B6 (H-2^b) mice³. Popliteal lymph node cells were fused to murine myeloma P3X63Ag.8.653 cells and hybridomas screened for antibodies specific to A-LAK cells.

Antibody 2F1 was used to show that KLRG1 is indirectly upregulated by expression of MHC class I molecules in an interaction affected by class I-specific Ly49 inhibitory receptors and was also used to show that KLRG1 expression is modulated by SHP-1 ³ . Additionally, 2F1 was used to identify the KLRG1 mouse homolog and show that it is not expressed on mouse mast cells ⁴.

E-, N-, R- cadherins

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8. Menendez CM, Jinkins JK, Carr DJ. J Immunol. 197(4):1262-1275. 2016.
9. Graham JB, Swarts JL, Lund JM. Curr Protoc Mouse Biol. 7(4):221-235. 2017.
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11. Yasuda K, Kitagawa Y, Kawakami R, et al. Nat Commun. 10(1):549. 2019.
12. Garg G, Muschaweckh A, Moreno H, et al. Cell Rep. 26(7):1854-1868.e5. 2019.
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14. Sidwell T, Liao Y, Garnham AL, et al. Nat Commun. 11(1):252. 2020.
15. Vasanthakumar A, Chisanga D, Blume J, et al. Nature. 579(7800):581-585. 2020.
16. Huang RY, Francois A, McGray AR, et al. Oncoimmunology. 6(1):e1249561. 2016.
17. Zhao M, Shao F, Yu D, et al. Nat Commun. 13(1):7600. 2022.