Recombinant Human Interleukin 15 Receptor, Alpha (IL-15Rα)

Interleukin 15 receptor, alpha (IL15RA) is a subunit of the IL-15 receptor and specifically binds to IL-15. It is expressed in a wide variety of T cells and B cells, as well as, non-lymphoid cells. The receptors of IL-15 and IL-2 share two subunits, the IL-2R beta and IL-2R gamma chains (1). This forms the basis of many overlapping biological activities of IL-15 and IL-2 (2). IL15RA is structurally related to IL2RA but is capable of binding IL-15 with high affinity independent of other subunits, which suggests the distinct roles between IL-15 and IL-2. This receptor is reported to enhance cell proliferation and expression of apoptosis inhibitor BCL2L1/BCL2-XL and BCL2 (3). The binding of IL-15 to IL15RA antagonizes the TNF-alpha-mediated apoptosis in fibroblasts by competing with TNRF1 for TRAF2 binding (4).

Fc Fusion Protein

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Ile31

Lyophilized

The predicted molecular weight of Recombinant Human IL-15 Rα is Mr 42.6 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 51-64 kDa.

42.6

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Giri, JG. et al. (1995) EMBO J. 14:3654
2. Anderson, DM. et al. (1995) J. Biol. Chem. 270:29862
3. Wu, TS. et al. (2002) Immunol. 168:705
4. Bulfone-Paus, S. et al. (1999) FASEB 13:1575