Recombinant Human Interleukin 15 Receptor, Alpha (IL-15Rα)

Recombinant Human Interleukin 15 Receptor, Alpha (IL-15Rα)

Product No.: I-543

[product_table name="All Top" skus="I-543"]

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Prod. No.I-543
Expression Host
sf Insect Cells

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Background

Interleukin 15 receptor, alpha (IL15RA) is a subunit of the IL-15 receptor and specifically binds to IL-15. It is expressed in a wide variety of T cells and B cells, as well as, non-lymphoid cells. The receptors of IL-15 and IL-2 share two subunits, the IL-2R beta and IL-2R gamma chains (1). This forms the basis of many overlapping biological activities of IL-15 and IL-2 (2). IL15RA is structurally related to IL2RA but is capable of binding IL-15 with high affinity independent of other subunits, which suggests the distinct roles between IL-15 and IL-2. This receptor is reported to enhance cell proliferation and expression of apoptosis inhibitor BCL2L1/BCL2-XL and BCL2 (3). The binding of IL-15 to IL15RA antagonizes the TNF-alpha-mediated apoptosis in fibroblasts by competing with TNRF1 for TRAF2 binding (4).

Protein Details

Purity
>95% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Fusion Protein Tag
Fc Fusion Protein
Protein Accession No.
Amino Acid Sequence
itcpppmsve hadiwvksys lysreryicn sgfkrkagts sltecvlnka tnvahwttps lkcikpaass pssnntaatt aaivpgsqlm pskspstgtt eisshesshg tpsqttaknw eltasashqp pgvypqghsd ttiegrdmdp kscdkthtcp pcpapellgg psvflfppkp kdtlmisrtp evtcvvvdvs hedpevkfnw yvdgvevhna ktkpreeqyn styrvvsvlt vlhqdwlngk eykckvsnka lpapiektis kakgqprepq vytlppsrde ltknqvsltc lvkgfypsdi avewesngqp ennykttppv ldsdgsffly skltvdksrw qqgnvfscsv mhealhnhyt qkslslspgk hhhhhh
N-terminal Sequence Analysis
Ile31
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human IL-15 Rα is Mr 42.6 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 51-64 kDa.
Predicted Molecular Mass
42.6
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Giri, JG. et al. (1995) EMBO J. 14:3654
2. Anderson, DM. et al. (1995) J. Biol. Chem. 270:29862
3. Wu, TS. et al. (2002) Immunol. 168:705
4. Bulfone-Paus, S. et al. (1999) FASEB 13:1575
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.