Recombinant Mouse NGF R

Nerve Growth Factor Receptor (NGFR p75, TNFRSF16) is a which binds NGF and other neurotrophins including BDNF, NT3, NT4/5 with similar, low affinity. NGFR p75 is mainly expressed in Schwann cells and neurons and in a variety of non-neuronal cells. NGFR p75 is necessary for regulating neuronal growth, migration, differentiation and cell death during development of the central and peripheral nervous system (1). The signal transduction mechanisms and components leading to NGFR p75 multiple signals are complex and not well understood (2).

The biological activity of Mouse NGF R was determined byits ability to inhibit rmβ-NGF-induced proliferation of TF-1 cells. The expected ED₅₀ 0.5 - 1.5 µg/ml in the presence of 2 ng/ml of rmβ-NGF.

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Gly20

Lyophilized

The predicted molecular weight of Recombinant Mouse NGFR is Mr 50.4 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 75-100 kDa.

50.4

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Barker, PA. et al. (1992) Mol. Cell. Biochem. 110:1
2. Bamji, AX. et al. (1998) J. Cell Biol. 140:911