Recombinant Mouse Osteoprotegerin

Osteoprotegerin (OPG), also known as osteoclastogenesis inhibitory factor (OCIF) is a cytokine, which can inhibit osteoclast differentiation, activity, and survival.¹ It is a member of the tumor necrosis factor (TNF) receptor superfamily.² OPG may provide a pharmacological tool for osteoporotic and erosive bone disorders.³

The biological activity of Mouse OPG was determined by its ability to inhibit TRAIL mediated cytotoxicity using L929 mouse fibrosarcoma cells treated with TRAIL. The expected ED₅₀ for this effect is 25-50 ng/ml in the presence of 50 ng/ml of recombinant human TRAIL.

Fc Fusion Protein

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Glu22

Lyophilized

The predicted molecular weight of Recombinant Mouse OPG is Mr 70.9 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 85-95 kDa.

70.9

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Kurihara, H. et al. (2002) Cytokine 18: 133
2. Hofbauer, LC. et al. (2002) Arteriosc. Throm. and Vasc. Biol. 22:549–553
3. Dunstan, CR. et al. (2001) J Bone Miner Res. 16: 348