Recombinant Human Osteoprotegerin (OPG)

Osteoprotegerin (OPG), also known as TNFRSF11B, is a cytokine and member of the TNF receptor superfamily that is expressed in a wide variety of tissues including adult heart, lung, kidney, liver, spleen, prostate, lymph node and bone marrow. OPG is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption.¹ It specifically binds to its ligand, osteoprotegerin ligand (OPGL), which is a key extracellular regulator of osteoclast development. Therefore, OPG may provide a pharmacological tool for osteoporotic and erosive bone disorders. Studies also suggest this protein and its ligand play a role in lymph-node organogenesis and vascular calcification.⁴

The biological activity of Human OPG was determined by its ability to neutralize apoptosis of mouse L-929 cells treated with 20 ng/ml cross-linked soluble rhTRAIL. The expected ED₅₀ for this effect is typically 8 - 24 ng/ml.

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Lyophilized

The predicted molecular weight of each monomer of mature OPG(generated after removal of a 21 amino acid signal peptide) is Mr 43.5 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE under reducing conditions, as a result of glycosylation, of recombinant Human OPG is Mr 55 kDa.

43.5

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Cher, ML. et al. (2007) Am J Pathol. 170(6):2100-11. PubMed
2. Schoppet, M. et al. (1980) Arteriosclerosis, Thrombosis, & Vascular Biol. 22:549
3. Kurihara, H. et al. (2002) Cytokine 18:133
4. Dunstan, CR. et al. (2001) J. Bone Miner. Res. 16:348
5. Collin-Osdoby, P. et al. (2004) Circulation Res. 95:1046