Antibodies and Reagents distributed by Caltag Medsystems
Leinco Technologies is a biotechnology company founded in 1992 as a specialty manufacturer of early discovery research products including antibodies, recombinant proteins, ELISA kits, second-step reagents, and other life sciences products. Shortly thereafter, we also established ourselves as a premier provider of custom R&D and manufacturing services focusing on monoclonal antibodies and recombinant proteins. Our innovative products and services are used to augment the early discovery process in life science research, diagnostics, and groundbreaking development of protein therapeutics.
Caltag Medsystems Ltd.
Whiteleaf Business Centre
11, Little Balmer
Buckingham
MK18 1TF
UK
Telephone: +44 (0)1280 827460 or 0800 279 9113
Sales and Customer Support: office@
*Products and services are for life science research use only. Products and services are in no way intended for use in diagnostic or therapeutic applications in humans.
In vivo Functional Antibodies
Antibodies can bind in a variety of ways, which has been exploited in in vivo diagnostics. In vivo antibodies from Leinco serve the purpose of research and are applied in living animals or cells, for cell depletion, activation, neutralization or blocking and many more. Monoclonal antibodies are primarily used here.
Leinco has proprietary methods to produce its functional in vivo antibodies in outstanding quality and highest purity standards. Two quality standards of in vivo antibodies are maintained, In vivo GOLDTM and In vivo PLATINUMTM.
Both series offer monoclonal in vivo antibodies with cross-reactivity for mouse and human species, among others.
GOLD™ vs. PLATINUM™ in vivo Antibody Quality Standards
| Specifications |  |  |
|---|---|---|
| Binding validation determined by WB, FC or ELISA | Yes | Yes |
| Endotoxin level determined by the LAL method | ≤ 1.0 EU/mg | ≤ 0.5 EU/mg |
| Antibody aggregation screening by analytical SEC | ≥ 95% monomer | ≥ 98% monomer |
| Purity by SDS PAGE | ≥ 95% | ≥ 98% |
| Formulation for in vivo use | No preservatives No stabilizers No carrier protein Sterile PBS pH 7.2 – No K+ or Ca2+ Concentration: > 5mg/mL | No preservatives No stabilizers No carrier protein Sterile PBS pH 7.2 – No K+ or Ca2+ Concentration: > 5mg/mL |
| Murine pathogen screening | Not applicable, see PLATINUM functional grade antibodies | Pathogen tested (IMPACT1) (see table) |
| Applications | In vivo functional studies and may be used for WB, FC, IF or IHC | |
| Product preparation | Functional grade preclinical antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. | |
| Storage and handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 1 month, 2 to 8 °C under sterile conditions, as supplied. • 12 months, -70 °C under sterile conditions. | |
IDEXX IMPACT I (PCR-based) Mouse Pathogen Profile for Purified In vivo PLATINUM™ Grade Antibodies
| Mycoplasma sp. | Mouse adenovirus 1 (MAV1) | Lactate dehydrogenase-elevating virus (LDEV) |
| Mycoplasma pulmonis | Mouse adenovirus 2 (MAV2) | Mouse kidney parvovirus (MKPV) |
| Sendai virus | Murine norovirus (MNV) | Hantaan Virus |
| Mouse hepatitis virus (MHV) | Reovirus 3 (REO3) | Mouse cytomegalovirus (mCMV) |
| Pneumonia virus of mice (PVM) | Mouse rotavirus (EDIM) | K virus |
| Minute virus of mice (MVM) | Ectromelia virus | Mouse thymic virus (MTV) |
| Mouse parvovirus (MPV) | Polyomavirus | Corynebacterium bovis |
| Theiler’s murine encephalomyelitis (TMEV) | Lymphocytic choriomeningitis virus (LCMV) | Corynebacterium sp. |
In vivo Cell Specific Depletion – T Cell, B Cell, NK Cells, and More
Leinco Technologies offers monoclonal antibodies to mouse cell specific surface proteins and cytokines for in vivo cell specific depletion studies. Depletion can occur naturally (i.e. in HIV) or be induced for treatment purposes. Therefore, it is a useful tool in therapeutic, immunotherapy and diagnostic research. It is common practice for researchers to use antibodies to remove a specific cell type to infer its function, and mouse cell depletion in vivo experiments are used to establish the role of specific cell types in a variety of immunological processes. As an example, in vivo T cell depletion experiments using a combination of anti-CD4 (Clone GK1.5) and anti-CD8 (Clone 2.43 or 53-5.8) antibodies are efficient and produce less deleterious effects on other blood cell populations compared to using CD3 depletion antibodies (like clone 145-2C11 or 17A2).
Immune Checkpoints
Leinco Technologies offers a wide range of well known immune checkpoint antibodies and proteins for use in cancer research. Leinco is the premier manufacturer of in vivo functional grade antibodies with the highest purity specifications available. When conducting immune checkpoint preclinical research, it is important to use high purity in vivo grade antibodies to generate reliable data. Please click the learn more button below to see why Leinco Technologies should be your choice for high purity immune checkpoint antibodies.
