CODEX® (CO-Detecting by indEXing) Introduction

Overcoming challenges of traditional multiplexed immunofluorescence staining has now become easier with the CODEX® ultrahigh multiplexed imaging system and assay reagents. This innovative, highly scalable technology targets subsets of an antibody panel through repetitive imaging cycles while revealing over 40 biomarkers in a single tissue section. The CODEX® technology was pioneered in Dr. Nolan’s lab at Stanford and now patented by Akoya Biosciences

The Benefits 

  • A comprehensive end-to-end solution containing the fluidics platform, assay reagents, and bioinformatics
  • Benchtop footprint that integrates with an existing fluorescence microscope
  • The system is scalable and capable of imaging up to 50 biomarkers per run
  • Preserves the sample for Region Of Interest (ROI) analysis and H&E staining
  • FF and FFPE tissue compatible

The Technology

CODEX Technology - Three components

Three main components of CODEX®

Multiplex imaging makes it possible to visualize various structures in a meaningful context in order to better our understanding of biological processes. Further, CODEX® makes use of DNA barcode technology comprised of unique oligonucleotide sequences conjugated to an antibody.

CODEX Technology - Tissue staining with antibody panel

Single-step staining to preserve sample integrity

The CODEX® fluidics instrument has an automated imaging cycle. The first step is the staining of tissue with to up to 50 uniquely barcoded antibodies, combined for a single tissue staining reaction.

CODEX workflow steps

An automated process of imaging biomarkers using the CODEX® instrument and a companion microscope

Then, applied to the tissue and imaged are three reporter dyes with complimentary barcodes. Finally, the three reporter dyes are removed and the process is repeated until all antibodies have been imaged.