ADAPT-3D™ Delipidation Buffer Component A

The ADAPT-3D™ Delipidation Buffer is formulated with a proprietary blend of reagents optimized for efficient lipid removal while preserving tissue integrity for high-resolution 3D imaging.

Store the buffer at room temperature in a dry environment, ensuring it is protected from moisture and temperature fluctuations. Do not freeze the product; when stored as directed, it maintains full potency and stability for up to six months.

Each lot of the ADAPT-3D™ Tissue Clearing Kit undergoes rigorous quality control testing at Leinco’s ISO 13485-certified facility. This includes verification of reagent composition, pH, clarity, and performance consistency using benchmark tissue samples. Our testing ensures that every kit delivers reliable clearing efficiency and reproducible results, supporting high-resolution imaging across various tissue types.

The ADAPT-3D Delipidation Buffer Component A is a crucial part of the innovative ADAPT-3D Tissue Clearing Kit developed by Leinco Technologies. This kit represents a significant advancement in preparing biological tissues for 3-dimensional fluorescence imaging, offering superior speed, versatility, and preservation of tissue morphology and fluorescence.

Traditional tissue clearing methods often involve lengthy protocols and can sometimes compromise the integrity of the tissue or the fluorescence signal. The ADAPT-3D method, including its specialized delipidation process, was designed to overcome these limitations. Lipids within tissues are a major source of light scattering, hindering deep tissue imaging. Efficient removal of these light-interfering substances is therefore paramount for achieving optical clarity and enabling clear visualization of fluorescent signals deep within samples.

ADAPT3D Delipidation Buffer Component A works in conjunction with Component B (and other buffers in the ADAPT-3D kit) to achieve partial delipidation. This targeted delipidation step is a key innovation of the ADAPT-3D protocol. It facilitates the removal of lipids while carefully maintaining the tissue's structural integrity and minimizing any potential loss of endogenous fluorophores or antibody staining efficiency. The development of this component is rooted in a deep understanding of the challenges associated with tissue transparency and the need for a robust, rapid, and non-toxic solution for advanced 3D microscopy.

By contributing to the efficient and gentle delipidation process, ADAPT3D Delipidation Buffer Component A plays a vital role in enabling researchers to rapidly prepare fixed tissue samples across a wide range of species and tissue types, from human intestinal tissue to intact mouse brains, for unprecedented depth and clarity in 3D fluorescence imaging.

Delipidation Step in the ADAPT-3D Protocol

Preparation: In a glass container, prepare the delipidation solution by mixing equal volumes (1:1) of ADAPT-3D™ Partial Delipidation Buffer A (Catalog #B672) and Partial Delipidation Buffer B (Catalog #B676).

Incubation: Submerge the samples in the freshly prepared ADAPT-3D™ Delipidation Buffer. Incubate until a moderate level of tissue transparency is achieved.

Important Safety Note: Partial Delipidation Buffer B is corrosive to most plastics. Always use glass containers and follow appropriate safety precautions. Consult the Safety Data Sheet (SDS) for detailed handling and storage information. Always wear protective gloves, lab coat, and safety goggles during this step.

Handle the ADAPT-3D™ Decolorization Buffer with care in all laboratory settings. Always use appropriate personal protective equipment, including gloves, lab coat, and safety goggles. For comprehensive guidance on safe handling, storage, and emergency procedures, refer to the product’s Safety Data Sheet (SDS). NOTE: Partial delipidization B is corrosive to most plastic types. Read the Safety Datasheet (SDS) and the following handling instructions. Wear appropriate protective eyewear, clothing and gloves.

At Leinco Technologies, a U.S.-based manufacturer headquartered in St. Louis, Missouri, we are committed to advancing disease diagnostics and treatment through high-performance immunoassay reagents. Our ISO 13485-certified facility ensures the highest standards of quality and consistency. Featuring our proprietary ADAPT-3D™ imaging technology, we offer a robust portfolio of premium reagents that empower assay developers to build sensitive, reliable, and reproducible immunoassays for applications ranging from ELISA to point-of-care diagnostics.