Recombinant Human ACE-2 (His-tag)

Angiotensin I converting enzyme 2 (ACE2) is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin[1-9].¹ ,or the conversion of angiotensin II to angiotensin 1-7.² ACE 2 has direct effects on cardiac function², and is expressed predominantly in vascular endothelial cells of the heart and the kidneys.³ ACE2 plays a regulatory role in lung pathophysiology, including pulmonary fibrosis and acute lung disease. Activation of ACE2 by a small molecule can be a therapeutically relevant approach for treating and controlling Pulmonary Hypertension (PH).⁴ ACE2 may also have clinical potential as a novel molecular target for the treatment of pancreatic ductal adenocarcinoma (PDAC).⁵

Histidine Tag

qst ieeqaktfld kfnheaedlf yqsslaswny ntniteenvq nmnnagdkws aflkeqstla qmyplqeiqn ltvklqlqal qqngssvlse dkskrlntil ntmstiystg kvcnpdnpqe clllepglne imansldyne rlwaweswrs evgkqlrply eeyvvlknem aranhyedyg dywrgdyevn gvdgydysrg qliedvehtf eeikplyehl hayvraklmn aypsyispig clpahllgdm wgrfwtnlys ltvpfgqkpn idvtdamvdq awdaqrifke aekffvsvgl pnmtqgfwen smltdpgnvq kavchptawd lgkgdfrilm ctkvtmddfl tahhemghiq ydmayaaqpf llrnganegf heavgeimsl saatpkhlks igllspdfqe dneteinfll kqaltivgtl pftymlekwr wmvfkgeipk dqwmkkwwem kreivgvvep vphdetycdp aslfhvsndy sfiryytrtl yqfqfqealc qaakhegplh kcdisnstea gqklfnmlrl gksepwtlal envvgaknmn vrpllnyfep lftwlkdqnk nsfvgwstdw spyadqsikv rislksalgd kayewndnem ylfrssvaya mrqyflkvkn qmilfgeedv rvanlkpris fnffvtapkn vsdiiprtev ekairmsrsr indafrlndn sleflgiqpt lgppnqppvs hhhhhhhhhh

No results obtained: Gln18 predicted

Solution

The predicted molecular weight of Recombinant Human ACE-2 is Mr 85 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 120 kDa.

85

This recombinant protein was 0.2 µm filtered and is supplied in a solution of sodium chloride (NaCl), zinc chloride (ZnCl₂), tris and glycerol.

Working aliquots of this recombinant protein solution are stable for up to six months at -20°C to -70°C in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at -20° to -70°C for three months without detectable loss of activity. For long-term storage, aliquot and freeze at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles.

Polar Packs

1. Acton, S. et al. (2000) Circ Res. 87: E1
2. Gamliel-Lazarovich, A. et al. (2007) Cardiovasc Res. 73: 463
3. Nabel, EG. et al. (2000) N Engl J Med. 347: 1795
4. Raizada, MK. et al. (2009) Am J Respir Crit Care Med. 179(11):1048-54.
5. Yuan, Y. et al. (2009) Tohoku J Exp Med. 217: 123