Recombinant Human Persephin

Persephin (PSP) is a secreted protein belonging to the glial cell line-derived neurotrophic factor (GDNF) family of the TGF-b superfamily. Just like factor GDNF and neurturin (NTN), PSP promotes the survival of ventral midbrain dopaminergic neurons and prevents their degeneration. Persephin also supports the survival of motor neurons.¹ Persephin is expressed at very low levels in most tissues. It is synthesized throughout the nervous system and has both astroglial and neuronal origin.²

The protein exhibits full biological activity when compared to standard controls. Analysis via a cell proliferation assay utilizing human TT medullary thyroid cancer cells demonstrated an ED50 of less than 10 ng/ml. This corresponds to a specific activity of greater than 1.0 × 100,000 IU/mg

ALSGPCQLWS LTLSVAELGL GYASEEKVIF RYCAGSCPRG ARTQHGLALA RLQGQGRAHG GPCCRPTRYT DVAFLDDRHR WQRLPQLSAA ACGCGG.

Lyophilized

The molecular weight of recombinant human PSPN produced in E. coli is approximately 20.5 kDa, representing a disulfide-linked homodimer comprised of two 96-amino-acid subunits.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Johnson EM et al. (1998) Neuron 20: 245 2. Krieglstein K. et al. (1998) J Neurosci Res. 53: 494