Recombinant Mouse Amphiregulin
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BackgroundAmphiregulin (AR) is a member of the EGF family of cytokines which is comprised of at least ten proteins including EGF, TGF-α, HB-EGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or several EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. Amphiregulin was originally isolated from the conditioned media of a PMA-treated MCF-7 human breast carcinoma cell line. The Amphiregulin mRNA expression can be detected in numerous carcinoma cell lines and the epithelial cells of various human tissues including colon, stomach, breast, ovary, kidney, etc. AR is also a growth inhibitor for various tumor cell lines. In certain colon carcinoma cell lines, AR has been shown to be an autocrine growth factor. Amphiregulin can bind to the EGF receptor. It has been suggested that in certain cell types, AR bioactivity may be mediated through the EGF receptor. Protein DetailsPurity >97% by SDS Page and analyzed by silver stain. Endotoxin Level <0.1 EU/µg as determined by the LAL method Biological Activity The biological activity of Mouse Amphiregulin was determined in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts. The expected ED<sub>50</sub> 5 - 20 ng/ml. Protein Accession No. Amino Acid Sequence Ser94 - Lys191 (Accession # Q4FJT2) N-terminal Sequence Analysis Ser94 State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Mouse Amphiregulin is Mr 11.3 kDa. Predicted Molecular Mass 11.3 Formulation This protein was 0.2 µm filtered and lyophilized from PBS. Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day Ambient NCBI Gene Bank Certificate of AnalysisIMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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