Recombinant Mouse Ret

The biological activity of Mouse Ret was determined by its binding ability in a functional ELISA. Serial dilution of immobilized rmRet (100 μl/well) was tested for binding with the GDNFGFRα1 complex using 4 ng/ml rhGDNF and 1 μg/ml rrGFRα1. The concentration of immobilized Ret that produces 50% of the optimal binding response is found to be approximately 2 - 5 μg/ml.

Fc Fusion Protein

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Leu29

Lyophilized

The predicted molecular weight of Recombinant Mouse Ret is Mr 95 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 150 kDa.

95

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient