Primary antibodies are immunoglobulins (monoclonal or polyclonal) developed to bind specific epitopes with high specificity on proteins, carbohydrates or small molecules for use in many assay platforms such as flow cytometry, ELISA, western blotting, fluorescent imaging, microscopy and others. Characteristics such as affinity, cross-reactivity, solubility, class and isotype are considered when selecting a primary antibody for a specific application. The primary antibodies developed and manufactured by Leinco Technologies are available purified or conjugated to enzymes or fluorescent dyes. Each antibody conjugate is optimized and tested in the intended applications for your use.
Monoclonal & Polyclonal Primary Antibody Differences
Polyclonal antibodies are a pool of antibodies originating from different B cells in a host animal that recognize more than one epitope on the same protein or antigen. In contrast, a monoclonal antibody is an antibody originating from a single B cell in a host animal that recognizes only one epitope or binding site on a protein or other antigen. Polyclonal antibodies are usually purified from the serum of an immunized host animal such as a rabbit, chicken or goat. Whereas monoclonal antibodies are usually purified from cell culture media supernatant from the growth of a hybridoma cell line. Monoclonal antibodies are divalent but monospecific. Polyclonal antibodies are also divalent but consist of a pool of antibodies with multiple epitope specificities.