ADAPT-3D™ Decolorization Buffer

The ADAPT-3D™ Decolorization Buffer is a specially formulated solution designed to remove light-interfering substances from tissue, enabling significantly clearer and deeper 3D imaging by optimizing the tissue's optical properties.

Store the buffer at room temperature in a dry environment, ensuring it is protected from moisture and temperature fluctuations. Do not freeze the product; when stored as directed, it maintains full potency and stability for up to six months.

Room temperature via Ground Shipping or 2-Day Air.

Each lot of the ADAPT-3D™ Tissue Clearing Kit undergoes rigorous quality control testing at Leinco’s ISO 13485-certified facility. This includes verification of reagent composition, pH, clarity, and performance consistency using benchmark tissue samples. Our testing ensures that every kit delivers reliable clearing efficiency and reproducible results, supporting high-resolution imaging across various tissue types.

The ADAPT-3D™ Decolorization Buffer is a crucial component of the ADAPT-3D (Accelerated Deep Adaptable Processing of Tissue for 3-Dimensional Fluorescence Tissue Imaging) method. In 3D tissue imaging, a significant challenge is the presence of light-interfering substances within the tissue, such as pigments and lipids, which can scatter light and hinder deep penetration and clear visualization. The Decolorization Buffer specifically addresses this by working to remove these light-absorbing and scattering molecules from the tissue. This process, often combined with delipidation (removal of lipids), makes the tissue more transparent and allows for superior light penetration during fluorescence microscopy. By effectively "decoloring" the tissue, the buffer enhances the signal-to-noise ratio and improves the overall quality and depth of 3D imaging, leading to clearer and more accurate representations of cellular structures and organization. This step is vital for achieving the rapid processing and high-quality results that ADAPT-3D aims to deliver.

To incorporate the ADAPT-3D™ Decolorization Buffer into your 3D tissue imaging protocol, follow this step:

Incubate the tissue samples in the ADAPT-3D™ Decolorization Buffer (catalog# B671) until partial transparency is achieved. This critical step effectively removes light-interfering substances, such as heme and lipids, which are essential for enhancing light penetration and image clarity in downstream 3D fluorescence microscopy. Note: This is a single step to the entire ADAPT-3D imaging process.

The ADAPT-3D™ Decolorization Buffer requires careful handling in your laboratory setting. Always wear appropriate protective eyewear, clothing, and gloves when working with this reagent. For detailed safety information, including handling, storage, and emergency protocols, please consult the Safety Data Sheet (SDS).

At Leinco, we are a leading U.S. manufacturer based in St. Louis, Missouri, we're dedicated to advancing disease diagnostics and treatment through premium immunoassay reagents, including our innovative ADAPT-3D™ 3D imaging process. Our ISO 13485-certified facility upholds the highest quality standards, guaranteeing consistent and reliable performance for all your immunoassay development needs. We offer a comprehensive suite of high-quality reagents, empowering assay developers to create consistent, reliable, and sensitive immunoassays for a wide range of applications, such as ELISA and point-of-care diagnostics.