Anti-Mouse CD317 [Clone 927] — Purified in vivo PLATINUM™ Functional Grade

Clone 927 is a monoclonal antibody commonly used in in vivo mouse studies to specifically deplete plasmacytoid dendritic cells (pDCs) and to block interferon-alpha (IFN-?) secretion by pDCs and other IFN-producing cells. Its target is murine CD317 (also known as BST2 or PDCA-1), a cell surface molecule predominantly expressed on these cell types.

Key uses and details:

• pDC Depletion: When administered in vivo, clone 927 effectively depletes pDCs by as much as ~95% in mice, which allows researchers to study the biological roles and downstream effects of these cells in immune responses.
• Functional Blockade: Administration of 927 also abrogates IFN-? secretion in response to stimuli such as CpG oligodeoxynucleotides, blocking a major functional output of pDCs and related IFN-producing cells.
• Experimental Applications: Clone 927 is utilized in experimental settings where selective removal or inactivation of pDCs or IFN-producing cells is desired, including studies of antiviral immunity, autoimmunity, and inflammation.
• Antigen Distribution: CD317 expression in the mouse is mostly restricted to IFN-producing cells and pDCs under normal conditions, but can be upregulated in other immune cell types upon stimulation (e.g. with IFNs, virus exposure, TLR ligands).

In summary, in vivo mouse studies use clone 927 to deplete pDCs and block type I interferon production, enabling mechanistic studies of immune cell function, especially relating to the innate response to pathogens and immunomodulation.

The correct storage temperature for sterile packaged clone 927 (anti-mouse CD317 monoclonal antibody) is 2–8°C when stored as received in sterile form for up to one month. For longer-term storage, aliquot the product aseptically without diluting and store at ? –70°C. Avoid repeated freeze-thaw cycles to preserve antibody integrity.

• Short-term (up to 1 month): 2–8°C
• Long-term: ? –70°C (after aliquoting, do not dilute)
• The antibody should be stored undiluted.
• Always avoid repeated freeze/thaw cycles.

These guidelines apply specifically to sterile, functional-grade clone 927 antibody, as per the manufacturer's recommendations. For any changes in storage or handling practices, consult the lot-specific datasheet, as they may be periodically updated.

Some of the most commonly used antibodies or proteins with clone 927 in the literature include surface markers and lineage-defining proteins typical of immune and dendritic cell profiling, as well as markers for specific cell depletion and functional assays.

Key antibodies or proteins that are often used in combination with 927 include:

• CD11c: A classical marker for dendritic cells; frequently used with 927 to help define plasmacytoid dendritic cell (pDC) populations or distinguish them from conventional DCs.
• B220: Also known as CD45R, this marker is commonly used to identify B cells and plasmacytoid dendritic cells; often paired with 927 in immunophenotyping panels for murine studies.
• Ly-6C: Utilized as a marker for distinguishing pDCs as part of multicolor flow cytometry panels.
• CD11b: Serves as an exclusion marker (pDCs are CD11b^? in mice); used to refine gating strategies in combination with 927.

In the generation and validation of clone 927, hybridomas were screened against cells that were CD11c^+ B220^+ Ly-6C^+ CD11b^? (splenocytes) to ensure specificity for murine CD317/BST2 (PDCA-1), which is the antigen recognized by 927.

In addition, 927 itself is commonly used for:

• Depletion of plasmacytoid dendritic cells in vivo
• Functional blockade of CD317/BST2
• Immunofluorescence and immunohistochemistry, frequently alongside the above markers

These antibody combinations and panels are widely reported in the immunology literature to characterize or experimentally deplete pDC populations and to study immune responses in various mouse models.

Key findings from scientific literature that cite "clone 927" primarily relate to its use as a monoclonal antibody targeting mouse CD317 (also known as BST2 or PDCA-1) and to its utility in the identification, depletion, and functional study of interferon-producing cells (IPCs), especially plasmacytoid dendritic cells (pDCs).

Key Findings:

• Clone 927 was developed to address difficulties in identifying and studying IPCs due to their rarity and complex phenotype in murine tissues.
• The antibody specifically recognizes CD317, which serves as a marker for IPCs (plasmacytoid dendritic cells) in mice under naïve (non-inflamed) conditions.
• CD317 expression on IPCs is typically independent of interferons (IFNs), but type I IFNs and IFN-? can induce CD317 on many other cell types.
• In vivo administration of clone 927:
  • Ablates IFN-? secretion by IPCs in response to CpG stimulation.
  • Depletes IPCs by approximately 95% in treated mice, which also leads to a significant reduction in plasma cells.
• CD317 is found both on the cell surface and within the Golgi apparatus of IPCs, participating in protein sorting and the immune response to viral stimulation.
• Clone 927 (rat IgG2b) was generated by immunizing rats with bone marrow-derived IPCs, then screening for antibodies recognizing CD11c+B220+Ly-6C+CD11b– splenocytes.

Broader Implications:

• Clone 927 has been instrumental in research exploring the role of IPCs/pDCs in innate and adaptive immune responses, and in experiments requiring the depletion or tracking of these cells.
• Its use allows for the study of pDC functions, depletion effects, and surface marker expression dynamics during infection, inflammation, or immune modulation.

Alternative "927" Usage:

• DJ-927, unrelated to clone 927/CD317, is a novel oral taxane drug tested for cancer therapy, notable for overcoming P-glycoprotein-mediated drug resistance. This is a distinct scientific context and not directly related to clone 927 antibody research.

In summary, clone 927 citations in scientific papers consistently highlight its pivotal role as a tool for identifying, depleting, and functionally analyzing mouse IPCs/pDCs, thus facilitating research in immunology and cell biology.