Anti-Mouse CD317 [Clone 927] — Purified in vivo PLATINUM™ Functional Grade
Anti-Mouse CD317 [Clone 927] — Purified in vivo PLATINUM™ Functional Grade
Product No.: C792
Clone 927 Target CD317 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, PDCA-1 Isotype Rat IgG2b κ Applications Depletion , FA , FC , ICC , IF Microscopy , in vivo |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Dilution Buffer Immunogen Mouse plasmacytoid dendritic cells Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco FC Additional Applications Reported In Literature ? In vivo depletion immunofluorescence microscopy functional assay Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 927 activity is directed against murine CD317 (BST2; PDCA-1). Background Monoclonal antibody (mAb) 927 recognizes CD317 (BST2; PDCA-1)1. CD317 is a specific marker of IFN-producing cells (IPCs aka plasmacytoid dendritic cells, DC) under naïve conditions. IPCs are early responders to viral infection and direct both the innate and adaptive immune response2,3. CD317 also promotes secretion in IPCs, presumably by sorting proteins between the Golgi apparatus and plasma membrane1. CD317 is located on the cell surface as well as intracellularly in the Golgi apparatus and is associated with lipid rafts.
CD317 is primarily present on the cell surfaces of murine IPCs in naïve mice, where its expression on resting and activated IPCs is independent of IFNs1. However, when stimulated with type I IFNs and IFN-γ, cell surface expression of CD317 is induced on most cell types. When administered in vivo, treatment with mAb 927 abrogates IFN-α secretion by IPCs in response to CpG as well as depletes IPCs ~ 95%, significantly reducing plasma cells. The 927 clone was generated to overcome barriers to the identification and study of IPCs caused by their scarcity in blood and tissues as well as their complex surface phenotype1. mAb 927 was generated by immunizing Wistar/CRL rats with bone marrow-derived IPCs with either CpG oligodeoxynucleotide 1826 or heat-killed Mycobacteria tuberculosis as adjuvant. Hybridoma lines were created by fusing popliteal lymph nodes with SP2/0 myeloma cells. Supernatants were screened for lines that recognize CD11c+B220+Ly-6c+CD11b-splenocytes. mAb 927 is rat IgG2b isotype. Antigen Distribution Murine CD317 is expressed by IFN-producing cells in naïve mouse spleen and by a wide variety of cell lines including T cells, mast cells, B cells, fibroblast cells, and pluripotent embryonal carcinoma cells. Additionally, mice challenged by influenza or other stimuli (CpG, LPS, murine CMV, poly(I:C), and imiquimod) express CD317 in DC and other myeloid cells as well as T cells, B cells, NKT cells, and some NK cells. CD317 is also expressed on CD138+ plasma cells in naïve mice and is upregulated by viral stimulation. Function Recently identified antiviral protein that blocks the release of nascent retrovirus or other particles from infected cells. NCBI Gene Bank ID UniProt.org Research Area Immunology Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. In Vivo Applications of Clone 927 in MiceClone 927 is a monoclonal antibody targeting murine CD317 (also known as BST2, tetherin, or PDCA-1), a cell surface glycoprotein expressed by plasmacytoid dendritic cells (pDCs), type I interferon (IFN)-producing cells (IPCs), and a variety of other immune cell types during inflammatory or infectious conditions. Key In Vivo Uses
Experimental Contexts
Technical Details
Summary Table
ConclusionClone 927 is a critical tool for in vivo mouse studies aiming to understand the roles of plasmacytoid dendritic cells, CD317, and type I interferon responses in health and disease. Its primary applications include targeted pDC depletion, IFN-α blockade, and functional investigation of CD317 in a broad range of immunological contexts. The antibody referred to as "927" in the context of the query is likely the Anti-Mouse CD317 (Clone 927), which is used to target plasmacytoid dendritic cells (pDCs). In the literature, this antibody is often used in combination with other markers to help define or distinguish pDC populations from conventional dendritic cells. Some commonly used antibodies or proteins in combination with CD317 include:
Other antibodies or proteins might be used in broader immunological studies, such as:
However, specific combinations directly mentioned alongside the "927" clone are focused on dendritic cell subsets like CD11c for defining cell populations. The term "clone 927" in scientific literature most commonly refers to a rat monoclonal antibody (mAb 927) that specifically targets murine CD317 (BST2; PDCA-1), which is a marker of plasmacytoid dendritic cells (pDCs or IFN-producing cells, IPCs) in mice. Here are the key findings from its use:
Summary Table: Key Applications and Findings
Alternative Interpretation: The dosing regimens for clone 927, an anti-mouse CD317 (BST2/PDCA-1) antibody, are not standardized across different mouse models. This clone is primarily used for depleting plasmacytoid dendritic cells (pDCs) in vivo, which is crucial for studying their role in immune responses and diseases. While specific dosing details are not uniformly documented for all mouse models, the general approach typically involves administering the antibody intraperitoneally. The choice of dosage may depend on the experimental design, including the desired level of pDC depletion and the specific immune response being studied. Empirical titration is often used to optimize the dose for each particular study, as the most effective dose can vary based on the model and research question. For example, in some studies, anti-PDCA-1 antibodies like clone 927 are used to deplete pDCs, but the exact dosing regimen may not be specified due to the variability in experimental designs and the need for tailored approaches in different mouse models. In summary, while the general application of clone 927 involves pDC depletion, the specific dosing regimens can vary widely depending on the research context and the mouse model used. References & Citations1. Blasius AL, Giurisato E, Cella M, et al. J Immunol. 177(5):3260-3265. 2006.
2. Colonna M, Trinchieri G, Liu YJ. Nat Immunol. 5(12):1219-1226. 2004. 3. Liu YJ. Annu Rev Immunol. 23:275-306. 2005. 4. Yun TJ, Lee JS, Machmach K, et al. Cell Metab. 23(5):852-866. 2016. 5. Toivonen R, Kong L, Rasool O, et al. J Immunol. 196(11):4750-4759. 2016. 6. Moniz RJ, Chan AM, Gordon LK, et al. FEMS Immunol Med Microbiol. 58(3):397-404. 2010. 7. Rajagopal D, Paturel C, Morel Y, et al. Blood. 115(10):1949-1957. 2010. 8. Nash WT, Gillespie AL, Brown MG. Front Immunol. 8:251. 2017. 9. Bradley KC, Finsterbusch K, Schnepf D, et al. Cell Rep. 28(1):245-256.e4. 2019. 10. Schliemann C, Roesli C, Kamada H, et al. Blood. 115(3):736-744. 2010. Technical ProtocolsDepletion FA  ICC IF Microscopy  Certificate of Analysis |
Formats Available
Prod No. | Description |
|---|---|
C791 | |
C799 | |
C785 | |
C786 | |
C783 | |
C784 | |
C792 | |
P420 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
