Anti-Mouse CD317 [Clone 927] — Purified in vivo GOLD™ Functional Grade

Anti-Mouse CD317 [Clone 927] — Purified in vivo GOLD™ Functional Grade

Product No.: C791

[product_table name="All Top" skus="D353"]

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Clone
927
Target
CD317
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, PDCA-1
Isotype
Rat IgG2b κ
Applications
Depletion
,
FA
,
FC
,
ICC
,
IF Microscopy
,
in vivo

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Recommended Isotype Controls
Recommended Dilution Buffer
Immunogen
Mouse plasmacytoid dendritic cells
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC
Additional Applications Reported In Literature ?
In vivo depletion
immunofluorescence microscopy
functional assay
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Clone 927 activity is directed against murine CD317 (BST2; PDCA-1).
Background
Monoclonal antibody (mAb) 927 recognizes CD317 (BST2; PDCA-1)1. CD317 is a specific marker of IFN-producing cells (IPCs aka plasmacytoid dendritic cells, DC) under naïve conditions. IPCs are early responders to viral infection and direct both the innate and adaptive immune response2,3. CD317 also promotes secretion in IPCs, presumably by sorting proteins between the Golgi apparatus and plasma membrane1. CD317 is located on the cell surface as well as intracellularly in the Golgi apparatus and is associated with lipid rafts.

CD317 is primarily present on the cell surfaces of murine IPCs in naïve mice, where its expression on resting and activated IPCs is independent of IFNs1. However, when stimulated with type I IFNs and IFN-γ, cell surface expression of CD317 is induced on most cell types. When administered in vivo, treatment with mAb 927 abrogates IFN-α secretion by IPCs in response to CpG as well as depletes IPCs ~ 95%, significantly reducing plasma cells.

The 927 clone was generated to overcome barriers to the identification and study of IPCs caused by their scarcity in blood and tissues as well as their complex surface phenotype1. mAb 927 was generated by immunizing Wistar/CRL rats with bone marrow-derived IPCs with either CpG oligodeoxynucleotide 1826 or heat-killed Mycobacteria tuberculosis as adjuvant. Hybridoma lines were created by fusing popliteal lymph nodes with SP2/0 myeloma cells. Supernatants were screened for lines that recognize CD11c+B220+Ly-6c+CD11b-splenocytes. mAb 927 is rat IgG2b isotype.
Antigen Distribution
Murine CD317 is expressed by IFN-producing cells in naïve mouse spleen and by a wide variety of cell lines including T cells, mast cells, B cells, fibroblast cells, and pluripotent embryonal carcinoma cells. Additionally, mice challenged by influenza or other stimuli (CpG, LPS, murine CMV, poly(I:C), and imiquimod) express CD317 in DC and other myeloid cells as well as T cells, B cells, NKT cells, and some NK cells. CD317 is also expressed on CD138+ plasma cells in naïve mice and is upregulated by viral stimulation.
NCBI Gene Bank ID
Research Area
Costimulatory Molecules
.
Immunology
.
Innate Immunity

Leinco Antibody Advisor

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Clone 927 is a monoclonal antibody that targets mouse CD317 (BST2, PDCA-1) and is most commonly used in vivo to specifically deplete plasmacytoid dendritic cells (pDCs) and block interferon-alpha (IFN-α) secretion by these and other IFN-producing cells in mice.

Key in vivo applications of clone 927 in mice include:

  • Depletion of plasmacytoid dendritic cells (pDCs): Clone 927 is highly effective at depleting pDCs (also known as IFN-producing cells or IPCs) from mouse tissues, achieving depletion rates of about 95%.
  • Functional blockade of IFN-α secretion: The antibody effectively blocks the secretion of interferon-alpha by pDCs and other IFN-producing cells, which is important for studying immune regulation and the role of these cytokines in various disease models.

Common experimental contexts for clone 927 in vivo:

  • Tumor immunology: Used to evaluate the role of pDCs and IFN-α in the tumor microenvironment and anti-tumor immunity.
  • Infectious disease models: Applied in mouse models of viral or bacterial infection to understand how pDCs and IFN responses modulate host defense and pathogenesis.
  • Autoimmunity research: Utilized in studies of autoimmune diseases to assess the impact of pDC depletion and IFN blockade on disease induction and progression.
  • Functional studies of immune cell populations: By depleting pDCs, researchers can dissect their specific contributions to immune responses in a variety of in vivo settings.

Additional notes:

  • Antigen distribution: CD317 (PDCA-1) is primarily a marker for pDCs under steady-state conditions but is also upregulated on many immune cell types following stimulation (e.g., via influenza, CpG, LPS, viral infection).
  • Exclusivity: While pDC depletion is the most prominent application, clone 927 can also impact other cells under certain inflammatory or viral challenge conditions where CD317 is induced on additional cell types.

In summary, clone 927 is a fundamental tool for in vivo depletion of plasmacytoid dendritic cells and inhibition of their IFN-α secretory function in mouse models, principally employed in studies of immunity, infection, cancer, and autoimmune diseases.

The antibody known as clone 927 targets mouse CD317 (also called BST2 or PDCA-1) and is widely used as a specific marker for plasmacytoid dendritic cells (pDCs) in mice. In the literature, several other antibodies and protein markers are commonly used in combination with 927, especially in immunophenotyping and depletion studies. The most frequently co-used markers are:

  • CD11c: A classical marker for dendritic cells, often used alongside 927 to better define pDC populations or distinguish pDCs from conventional dendritic cells (cDCs).
  • B220 (CD45R): Frequently combined in flow cytometry panels to identify pDCs as CD317+ B220+ cells; helps differentiate pDCs from other B cells and dendritic cell subsets (inferred from common immunological protocols).
  • CD19: Used to exclude B cells in gating strategies when characterizing pDCs with CD317/927 antibody (inferred from published gating strategies).
  • Siglec-H: Another pDC marker sometimes used with 927 for greater specificity and confirmation of pDC identity (inferred from common use in the field).

Key proteins and antibodies typically encountered with 927:

  • CD11c — Distinguishes between pDCs and other dendritic cell types.
  • B220 (CD45R) — Confirms hematopoietic origin and helps define pDCs within lymphoid organs.
  • Siglec-H — Additional marker confirming pDC identity.
  • CD19 and/or CD3 — Used to exclude B cells and T cells, respectively, in flow cytometry sorting panels and analyses.

These combinations are standard in flow cytometry and cell depletion experiments for mouse immune system studies, especially when focusing on the selective identification, quantification, or depletion of pDCs.

If your context is outside mouse immunology or pDC identification, please clarify for more tailored combinations.

The key findings from scientific literature on clone 927 mainly concern its use as a monoclonal antibody to identify and deplete murine plasmacytoid dendritic cells (pDCs), also referred to as interferon-producing cells (IPCs), by targeting the cell surface marker CD317 (BST2, PDCA-1).

Key findings include:

  • Specific Recognition and Depletion: Clone 927 specifically recognizes CD317, a marker for murine pDCs under naïve conditions, allowing for their identification and depletion in experimental models. Administration of clone 927 in vivo depletes pDCs by approximately 95% and abrogates IFN-α secretion by IPCs in response to CpG stimulation, thereby significantly reducing plasma cells and type I interferon production.

  • Research Applications: The clone facilitates mechanistic studies in tumor immunology, infection, and autoimmunity by enabling targeted depletion of pDCs, which are early responders to viral infection and crucial in directing both innate and adaptive immune responses.

  • Mechanism and Specificity: CD317 is present mainly on the cell surface of IPCs in naïve mice, with expression on both resting and activated IPCs being independent of interferons. However, upon stimulation with type I IFNs and IFN-γ, CD317 expression is induced on most cell types.

  • Development and Screening: Clone 927 was developed to address difficulties in identifying and studying IPCs due to their rarity and complex phenotype in murine tissues. The antibody is a rat IgG2b isotype, generated by immunizing rats with bone marrow-derived IPCs.

  • Broader Relevance of CD317: CD317 (BST2/tetherin) is expressed on a variety of immune cells, including T cells, B cells, mast cells, NK cells, and is involved in B-cell development and functions related to virus restriction, such as inhibiting retrovirus release—including HIV-1—by causing retention of virions, which can impact viral pathogenesis and therapeutic strategies.

Summary Table: Biological Role and Research Utility of Clone 927

AspectKey Finding
Target AntigenCD317 (BST2, PDCA-1, tetherin)
Main ApplicationIn vivo depletion of murine pDCs to study immune function
Mechanistic Effect~95% depletion of pDCs, abrogation of IFN-α secretion, reduction of plasma cells
Research UtilityTumor immunology, infection, autoimmunity, mechanistic studies of innate/adaptive immunity
CD317 ExpressionOn IPCs/pDCs in naïve mice; induced on many cell types upon interferon stimulation
Broader CD317 RoleRestricts viral release (e.g., HIV-1), involved in B-cell development and stromal cell interactions
Development ContextDeveloped to address difficulty in identifying/studying rare IPCs in murine tissue

In summary, citations for clone 927 focus on its essential role in selectively identifying and depleting murine pDCs via CD317 for immunological research, thereby enabling critical mechanistic insights into innate immunity and viral responses.

Dosing regimens of clone 927 (anti-mouse CD317/BST2, also known as PDCA-1) in mouse models typically involve intraperitoneal (IP) injection of 200–250 μg per mouse, given 2–3 times per week. These regimens are commonly used for depletion of plasmacytoid dendritic cells (pDCs) in vivo.

Key context and details:

  • There is no universally standardized protocol for clone 927; the dosing may be adjusted based on the experimental goal, mouse strain/model, and the outcome to be studied.
  • Most published protocols and product recommendations cite 200–250 μg IP injection per dose, with the frequency of 2–3 times weekly (e.g., every 2–3 days).
  • This approach is effective for robust depletion of pDCs (over 90%) and abrogation of IFN-α secretion in response to stimuli such as CpG.
  • Regimen details often do not vary significantly between major mouse models (e.g., C57BL/6, BALB/c), but doses might be fine-tuned in specific disease contexts or with co-administered agents. Direct comparisons of dose optimization across mouse strains are rarely published.
  • In influenza and infection models, this regimen effectively depletes targeted populations, though occasional studies may alter dose or frequency depending on particular experimental requirements.
  • The isotype of clone 927 is rat IgG2b, and it is often used in combination with other immunological interventions for mechanistic studies.

Summary table: Typical clone 927 dosing in mouse models

Mouse Model UseDose per MouseRouteFrequencyReference
pDC depletion/infection200–250 μgIntraperitoneal2–3x per week

Published studies and antibody suppliers suggest starting at 200–250 μg IP, 2–3 times weekly, and adjusting only as necessary for model-specific results. No major deviations in regimen have been described for specific mouse strains or disease models; reported adjustments are experimental rather than standard.

References & Citations

1. Blasius AL, Giurisato E, Cella M, et al. J Immunol. 177(5):3260-3265. 2006.
2. Colonna M, Trinchieri G, Liu YJ. Nat Immunol. 5(12):1219-1226. 2004.
3. Liu YJ. Annu Rev Immunol. 23:275-306. 2005.
4. Yun TJ, Lee JS, Machmach K, et al. Cell Metab. 23(5):852-866. 2016.
5. Toivonen R, Kong L, Rasool O, et al. J Immunol. 196(11):4750-4759. 2016.
6. Moniz RJ, Chan AM, Gordon LK, et al. FEMS Immunol Med Microbiol. 58(3):397-404. 2010.
7. Rajagopal D, Paturel C, Morel Y, et al. Blood. 115(10):1949-1957. 2010.
8. Nash WT, Gillespie AL, Brown MG. Front Immunol. 8:251. 2017.
9. Bradley KC, Finsterbusch K, Schnepf D, et al. Cell Rep. 28(1):245-256.e4. 2019.
10. Schliemann C, Roesli C, Kamada H, et al. Blood. 115(3):736-744. 2010.
Depletion
FA
Flow Cytometry
ICC
IF Microscopy
in vivo Protocol

Certificate of Analysis

Formats Available

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.