Anti-Mouse CD317 [Clone 927] — Purified in vivo GOLD™ Functional Grade
Anti-Mouse CD317 [Clone 927] — Purified in vivo GOLD™ Functional Grade
Product No.: C791
Clone 927 Target CD317 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, PDCA-1 Isotype Rat IgG2b κ Applications Depletion , FA , FC , ICC , IF Microscopy , in vivo |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Dilution Buffer Immunogen Mouse plasmacytoid dendritic cells Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.1 EU/µg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco FC Additional Applications Reported In Literature ? In vivo depletion immunofluorescence microscopy functional assay Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 927 activity is directed against murine CD317 (BST2; PDCA-1). Background Monoclonal antibody (mAb) 927 recognizes CD317 (BST2; PDCA-1)1. CD317 is a specific marker of IFN-producing cells (IPCs aka plasmacytoid dendritic cells, DC) under naïve conditions. IPCs are early responders to viral infection and direct both the innate and adaptive immune response2,3. CD317 also promotes secretion in IPCs, presumably by sorting proteins between the Golgi apparatus and plasma membrane1. CD317 is located on the cell surface as well as intracellularly in the Golgi apparatus and is associated with lipid rafts.
CD317 is primarily present on the cell surfaces of murine IPCs in naïve mice, where its expression on resting and activated IPCs is independent of IFNs1. However, when stimulated with type I IFNs and IFN-γ, cell surface expression of CD317 is induced on most cell types. When administered in vivo, treatment with mAb 927 abrogates IFN-α secretion by IPCs in response to CpG as well as depletes IPCs ~ 95%, significantly reducing plasma cells. The 927 clone was generated to overcome barriers to the identification and study of IPCs caused by their scarcity in blood and tissues as well as their complex surface phenotype1. mAb 927 was generated by immunizing Wistar/CRL rats with bone marrow-derived IPCs with either CpG oligodeoxynucleotide 1826 or heat-killed Mycobacteria tuberculosis as adjuvant. Hybridoma lines were created by fusing popliteal lymph nodes with SP2/0 myeloma cells. Supernatants were screened for lines that recognize CD11c+B220+Ly-6c+CD11b-splenocytes. mAb 927 is rat IgG2b isotype. Antigen Distribution Murine CD317 is expressed by IFN-producing cells in naïve mouse spleen and by a wide variety of cell lines including T cells, mast cells, B cells, fibroblast cells, and pluripotent embryonal carcinoma cells. Additionally, mice challenged by influenza or other stimuli (CpG, LPS, murine CMV, poly(I:C), and imiquimod) express CD317 in DC and other myeloid cells as well as T cells, B cells, NKT cells, and some NK cells. CD317 is also expressed on CD138+ plasma cells in naïve mice and is upregulated by viral stimulation. NCBI Gene Bank ID UniProt.org Research Area Costimulatory Molecules . Immunology . Innate Immunity Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Use of Clone 927 in In Vivo Mouse StudiesClone 927 is a rat IgG2b, ? monoclonal antibody specific for murine CD317 (also known as BST2 or PDCA-1), a type II transmembrane glycoprotein. Its primary application in in vivo mouse studies is for the specific depletion of plasmacytoid dendritic cells (pDCs), which are the main cell type expressing CD317 in naïve mice. Heres how clone 927 is utilized: Mechanism and Applications
Technical Details
Summary Table
ConclusionClone 927 is a well-validated, highly specific tool for depleting plasmacytoid dendritic cells in mice in vivo, enabling researchers to dissect the role of these cells in immunity and disease with minimal perturbation to other cell types. Its use is standard in studies requiring selective pDC ablation to understand their contributions to antiviral responses, tumor immunology, and autoimmune processes. Based on the product information provided, the correct storage temperature for sterile packaged Clone 927 (Anti-Mouse CD317 antibody) is 2-8°C (35-46°F). Storage GuidelinesShort-term storage: The functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. Long-term storage: For longer term storage, the antibody should be aseptically aliquoted in working volumes without diluting and stored at ? -70°C (-94°F). It's important to avoid repeated freeze-thaw cycles to maintain antibody integrity. The antibody solution should be stored undiluted at the recommended temperature range to preserve its functionality. This temperature range is standard for most antibody products and ensures optimal stability while preventing degradation that could occur at higher temperatures or damage from freezing at standard refrigeration temperatures. Based on the available information, clone 927 is an anti-mouse CD317 monoclonal antibody that recognizes CD317 (also known as BST2, PDCA-1, or tetherin) on plasmacytoid dendritic cells and other cell types. However, the search results do not provide specific information about other antibodies or proteins commonly used in combination with the 927 antibody in research applications. Primary Applications of Clone 927The 927 antibody is primarily used for studying interferon-producing cells (IPCs), also known as plasmacytoid dendritic cells, which are early responders to viral infection. The antibody has been validated for several applications including flow cytometry, immunofluorescence microscopy, functional assays, and in vivo depletion studies. Target Expression and FunctionCD317, the target antigen of clone 927, is expressed on various cell types including T cells, mast cells, B cells, fibroblast cells, and pluripotent embryonal carcinoma cells. The protein functions as an antiviral factor that blocks the release of nascent retrovirus particles from infected cells. When mice are challenged with stimuli such as influenza, CpG, LPS, or other immune activators, CD317 expression extends to dendritic cells, myeloid cells, T cells, B cells, NKT cells, and some NK cells. Potential Research ContextWhile specific combinations aren't detailed in the search results, the 927 antibody would likely be used alongside other markers for comprehensive immunophenotyping studies. Given that it targets plasmacytoid dendritic cells, researchers might combine it with other dendritic cell markers, B cell markers (since it's also expressed on CD138+ plasma cells), or interferon pathway-related proteins to study immune responses to viral infections or inflammatory conditions. The information available does not provide a comprehensive list of commonly co-used antibodies or proteins with the 927 clone in published literature. Key Findings from Clone 927 Scientific CitationsClone 927 most prominently refers to a monoclonal antibody (anti-mouse CD317, Clone 927) used primarily in immunology research. The scientific literature and product documentation highlight several critical findings and applications related to this clone: Primary Applications and Biological Insights
Technical and Experimental Details
Summary Table
ConclusionClone 927 is a critical reagent for the study of murine plasmacytoid dendritic cells and interferon responses, enabling researchers to identify, isolate, and functionally manipulate these rare but important immune cells. Its development has addressed significant technical barriers in IPC research, providing a robust tool for both basic and translational immunology studies. References & Citations1. Blasius AL, Giurisato E, Cella M, et al. J Immunol. 177(5):3260-3265. 2006.
2. Colonna M, Trinchieri G, Liu YJ. Nat Immunol. 5(12):1219-1226. 2004. 3. Liu YJ. Annu Rev Immunol. 23:275-306. 2005. 4. Yun TJ, Lee JS, Machmach K, et al. Cell Metab. 23(5):852-866. 2016. 5. Toivonen R, Kong L, Rasool O, et al. J Immunol. 196(11):4750-4759. 2016. 6. Moniz RJ, Chan AM, Gordon LK, et al. FEMS Immunol Med Microbiol. 58(3):397-404. 2010. 7. Rajagopal D, Paturel C, Morel Y, et al. Blood. 115(10):1949-1957. 2010. 8. Nash WT, Gillespie AL, Brown MG. Front Immunol. 8:251. 2017. 9. Bradley KC, Finsterbusch K, Schnepf D, et al. Cell Rep. 28(1):245-256.e4. 2019. 10. Schliemann C, Roesli C, Kamada H, et al. Blood. 115(3):736-744. 2010. Technical ProtocolsDepletion FA  ICC IF Microscopy  Certificate of Analysis |
Formats Available
Prod No. | Description |
|---|---|
C791 | |
C799 | |
C785 | |
C786 | |
C783 | |
C784 | |
C792 | |
P420 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
