Anti-Mouse Jagged 2 – Biotin

CHO derived Mouse Jag2

This Biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Liquid

This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.

Research Use Only

2 – 8° C Wet Ice

HMJ2-1 activity is directed against mouse Jagged2.

The Notch pathway is an intercellular signaling mechanism that controls cell fate and differentiation during embryonic and postnatal development^1,2,3. The pathway is highly conserved across species. Four mammalian Notch receptors (Notch 1-4) have been identified and can be activated by their ligands Jagged1, Jagged2, DLL1, DLL3, or DLL4. These ligands are transmembrane proteins that contain a Delta/Serrate/Lag-2 extracellular domain that is used for interactions with the Notch receptors³. Receptor-ligand engagement triggers cleavage of the Notch receptor extracellular domain, leading to the release of the Notch intracellular domain which travels to the nucleus and affects target gene expression. Dysregulation of Notch signaling affects inflammation³ as well as tumorigenesis and cancer growth^1,4.

HMJ2-1 was generated using CHO cells expressing murine Jagged2⁵.

Jagged2 is expressed by stromal and thymic lymphoid cells, splenic macrophages, and dendritic cells. Jagged2 can also be expressed by cancer stem cells and is upregulated on antigen-presenting cells after transplantation.

Notch receptors

1. Fazio C, Ricciardiello L. Cell Death Dis. 7(12):e2515. 2016.
2. https://www.ncbi.nlm.nih.gov/gene/3714
3. Sekine C, Nanki T, Yagita H. Arthritis Rheumatol. 66(10):2751-2761. 2014.
4. Mandula JK, Sierra-Mondragon RA, Jimenez RV, et al. Immunity. 57(5):1124-1140.e9. 2024.
5. Moriyama Y, Sekine C, Koyanagi A, et al. Int Immunol. 20(6):763-773. 2008.
6. Sekine C, Moriyama Y, Koyanagi A, et al. Int Immunol. 21(3):295-301. 2009.
7. Sekine C, Koyanagi A, Koyama N, et al. Arthritis Res Ther. 14(2):R45. 2012.
8. Kijima M, Yamaguchi T, Ishifune C, et al. Proc Natl Acad Sci U S A. 105(19):7010-7015. 2008.
9. Riella LV, Yang J, Chock S, et al. Eur J Immunol. 43(6):1449-1458. 2013.
10. Steinbuck MP, Arakcheeva K, Winandy S. J Immunol. 200(3):997-1007. 2018.