Mouse IgG1 Isotype Control [Clone HKSP84] — Purified in vivo PLATINUM™ Functional Grade
Mouse IgG1 Isotype Control [Clone HKSP84] — Purified in vivo PLATINUM™ Functional Grade
Product No.: P382
Clone HKSP84 Formats AvailableView All Product Type Isotype Control Isotype Mouse IgG1 κ Applications in vivo |
Antibody DetailsProduct DetailsHost Species Mouse Recommended Dilution Buffer Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C Working Concentration This isotype control antibody should be used at the same concentration as the primary antibody. RRIDAB_2831655 Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This Mouse IgG1 isotype control antibody (Anti-KLH) has been tested against selected species' cells and tissues to assure minimal cross reactivity. Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone HKSP84 is used as a Mouse IgG1 isotype control antibody in in vivo mouse studies to serve as a negative control, enabling researchers to distinguish specific effects of a test antibody from non-specific or background responses. HKSP84 is specifically designed so that:
In vivo applications include:
This rigorous use of HKSP84 ensures the reliability and interpretability of immunological data in mouse studies by controlling for background and non-specific responses. The antibody is formulated for stability, purity, and minimal endotoxin levels to avoid confounding immune activation or toxicity in experimental animals. In the literature, HKSP84 is primarily used as a mouse IgG1 isotype control antibody. Commonly, it is paired with other primary antibodiesespecially those raised in mousewhen researchers need to verify that observed effects are specific to their target and not due to nonspecific binding of mouse IgG1 antibodies. Isotype controls like HKSP84 are not typically paired with other specific proteins, but rather are used alongside a range of experimental antibodies in immunology, oncology, and cell biology studies. When HKSP84 is used, typical associated reagents or proteins include:
For functional or multiplexed assays, researchers would typically combine HKSP84 with:
In summary, HKSP84 is used with:
These combinations enable rigorous experimental controls for specificity and background, central to most immunological and cell biological studies. Clone HKSP84 is most commonly cited in the scientific literature as a mouse IgG1 isotype control antibody used for in vivo and immunological research applications, rather than for direct biological function or disease studies. There are no search results identifying clone HKSP84 itself in relation to specific experimental findings or disease associations. Key findings and usage from scientific literature and product documentation:
The available search results and product documentation consistently characterize HKSP84 as a negative control essential for validating experimental results and distinguishing true biological signal from isotype-related background. They do not report direct experimental or clinical findings about the clone itself. No citations were found linking "clone HKSP84" to mechanistic findings, disease models, or significant scientific discoveries beyond its critical role in antibody validation and experimental control. If you meant a different "clone HKSP84" in another biological or disease context, please clarify, as the above findings pertain specifically to the antibody isotype control widely used in immunological studies. Published dosing regimens for clone HKSP84 specifically in mouse models are not found in publicly available sources as of June 2024, and no peer-reviewed data detail its dose range, interval, or application across different models such as syngeneic tumors, humanized mice, or autoimmunity contexts. For context:
If HKSP84 is functionally or structurally similar to well-known checkpoint antibodies (such as RMP1-14 or 10F.9G2), initial dosing regimens could plausibly follow the above standards: 100–500 ?g per mouse, intraperitoneally, every 3–4 days. However, precise regimens must be empirically determined for each mouse model due to differences in antibody clearance, target expression, and immunogenicity. Regimen differences across models:
Note: Without direct evidence for HKSP84, these inferences are based on established protocols for analogous murine antibodies. If precise HKSP84 dosing information is required, consultation of unpublished experimental reports or contacting the supplier directly is recommended, as usage may be subject to restricted or proprietary data. If additional details about HKSP84's mechanism or target become available, dosing optimization should also consider:
In summary, dosing regimens for clone HKSP84 across mouse models remain unreported, but analogous murine antibodies follow standardized approaches ranging 100–500 ?g per mouse every 3–4 days, tailored to the experimental model and antibody characteristics. References & Citations |
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