Mouse IgG1 Isotype Control [Clone HKSP84] — Purified in vivo PLATINUM™ Functional Grade
Mouse IgG1 Isotype Control [Clone HKSP84] — Purified in vivo PLATINUM™ Functional Grade
Product No.: P382
Clone HKSP84 Formats AvailableView All Product Type Isotype Control Isotype Mouse IgG1 κ Applications in vivo |
Antibody DetailsProduct DetailsHost Species Mouse Recommended Dilution Buffer Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C Working Concentration This isotype control antibody should be used at the same concentration as the primary antibody. RRIDAB_2831655 Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This Mouse IgG1 isotype control antibody (Anti-KLH) has been tested against selected species' cells and tissues to assure minimal cross reactivity. Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone HKSP84 is most commonly used in in vivo applications in mice as an IgG1 isotype control antibody, primarily serving as a negative control to distinguish nonspecific background effects from true signals in immunological assays and therapeutic antibody studies. Key in vivo applications include:
Details relevant to in vivo use:
In summary, clone HKSP84 is an industry-standard in vivo isotype control for mouse IgG1 monoclonal antibody applications, indispensable for ensuring specificity, reliability, and interpretability in preclinical mouse immunological studies. Antibodies and Proteins Commonly Used with HKSP84Mouse IgG1 Isotype Control Clone HKSP84 (HKSP84) is widely used in immunological research as a negative control to determine the background staining or non-specific binding in experiments, especially when paired with primary or therapeutic antibodies. While the available literature does not provide specific studies or publications directly citing HKSP84, standard experimental practices and manufacturer guidance offer insight into typical applications and pairings. Typical Pairings in Immunological Assays
Broader Context: Isotype Controls in Immunology
Experimental Workflow
SummaryWhile there are no high-profile, peer-reviewed studies in the current search results that specifically detail the use of HKSP84 with particular proteins or antibodies, standard immunological practice indicates that HKSP84 is routinely paired with antibodies against common immune cell markers, intracellular signaling molecules, and cytokines in both in vitro and in vivo settings. It is also used alongside secondary antibodies for detection and in multiplexed assay formats. For precise experimental protocols, consulting the antibody manufacturer’s recommendations and relevant literature is advised. Based on the available information, clone HKSP84 lacks substantial direct citations in the scientific literature despite being a commercially available research reagent. The search results indicate that HKSP84 is a mouse IgG1 isotype control antibody, but do not provide specific published studies, experimental findings, or key scientific discoveries directly attributable to this clone. Characteristics of Clone HKSP84Clone HKSP84 is an anti-Hen Egg Lysozyme (HEL) antibody that serves as a mouse IgG1 isotype control. The immunogen used to generate this antibody is Trinitrophenol conjugated to Keyhole Limpet Hemocyanin (TNP-KLH), where TNP is a hapten not naturally expressed on human, mouse, rat, or non-human primate cells. This design ensures minimal cross-reactivity with experimental subjects, making it suitable as a negative control in immunological studies. The antibody has been tested against selected species' cells and tissues to verify minimal cross-reactivity. It is manufactured in animal-free facilities using in vitro cell culture techniques and purified through multi-step affinity chromatography with Protein A or G to achieve extremely low endotoxin levels, typically less than 1.0 EU/mg in standard formulations. Application as an Isotype ControlWhile specific research findings using HKSP84 are not documented in the search results, isotype controls like this serve a critical role in experimental design. They are used as negative controls to determine the specificity of primary antibodies in techniques such as ELISA, flow cytometry, and in vivo studies. The lack of published literature specifically citing HKSP84 suggests it functions primarily as a standardized control reagent rather than as an investigative tool that generates novel scientific findings. For researchers seeking specific experimental protocols or dosing regimens for HKSP84, consultation of original literature or direct contact with manufacturers would be necessary, as this information is not available in the current search results. There is no direct, peer-reviewed information specifying dosing regimens of clone HKSP84 across different mouse models in the available search results. Most data concern the general applications of clone HKSP84 as a mouse IgG1 isotype control antibody, commonly used to measure background effects in immunological assays and in vivo experiments. Essential context and details:
Key considerations for dosing variation:
If using HKSP84 in a new model:
Summary table (based on current best practices for murine IgG1 isotype controls):
Conclusion: References & Citations |
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