Mouse IgG1 Isotype Control [Clone HKSP] — Purified in vivo PLATINUM™ Functional Grade
Mouse IgG1 Isotype Control [Clone HKSP] — Purified in vivo PLATINUM™ Functional Grade
Product No.: M1411
Clone HKSP Formats AvailableView All Product Type Isotype Control Isotype Mouse IgG1 κ Applications FC , in vivo |
Antibody DetailsProduct DetailsHost Species Mouse Recommended Dilution Buffer Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C Working Concentration This isotype control antibody should be used at the same concentration as the primary antibody. RRIDAB_2831344 Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This Mouse IgG1 isotype control antibody (anti-BTV) has been tested against selected species' cells and tissues to assure minimal cross reactivity. This antibody was also pathogen tested and third-party certified by IDEXX BioReseach to meet the lowest mycoplasma specification and free of any viral pathogens of concern. Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. In Vivo Applications of Clone HKSP in MiceClone HKSP is a mouse IgG1 isotype control antibody widely used in immunological research, particularly in mouse models. Its primary in vivo application is not for direct therapeutic or experimental intervention but rather as a negative control to validate the specificity of experimental antibodies and account for potential nonspecific effects mediated by the Fc portion of antibodies. Key Uses in Mouse Studies
Research ContextWhile clone HKSP itself does not have a therapeutic role, its use is critical in the validation and interpretation of in vivo experiments involving antibody-based interventions. For example, in cancer immunotherapy, gene therapy, or immune cell engineering studies in mice, appropriate isotype controls like HKSP are essential to demonstrate that observed outcomes are genuinely due to the experimental treatment. Advanced In Vivo Applications (Non-HKSP Related)Recent advances in mouse in vivo research—such as in vivo gene editing of T cells for enhanced cancer immunotherapy or hematopoietic stem cell (HSC) gene therapy for blood disorders—often employ sophisticated targeting strategies. However, these studies typically use experimental antibodies or genetic tools, not isotype controls like HKSP. HKSP’s role remains rooted in quality control and experimental rigor, ensuring that novel findings are not confounded by nonspecific antibody effects. Summary Table
Clone HKSP is not used for direct therapeutic or experimental intervention but is indispensable for establishing the specificity and reliability of antibody-based experiments in mice. Based on the available search results, there appears to be confusion between two different research areas that use similar abbreviations but refer to entirely different subjects. HKSP as an Isotype ControlWhen HKSP refers to a mouse IgG1 isotype control clone, it is not typically paired with other antibodies or proteins because it serves as a negative control in experimental design. As an isotype control, clone HKSP is used exclusively to validate the specificity of experimental antibodies in in vivo studies in mice, ensuring minimal cross-reactivity with cells and tissues. The search results emphasize that HKSP as an isotype control has no functional antibodies or clones with biological or therapeutic significance—it is exclusively cited as a control in experimental design. Therefore, there are no commonly paired antibodies or proteins with HKSP in this context, as its purpose is to serve as a baseline comparison rather than an active research tool. KSP (Kinesin Spindle Protein) InhibitorsThe search results do mention KSP inhibitors in antibody-drug conjugate (ADC) research, which is a completely separate area from HKSP immunology studies. In this cancer therapeutics context, KSP inhibitor payloads are commonly paired with:
However, these KSP-related findings should not be conflated with HKSP immune response research, as they represent entirely different scientific contexts. Based on the available search results, specific key findings from clone HKSP citations in scientific literature are not clearly defined. The information about clone HKSP is limited to its technical application rather than substantive research findings. Technical FunctionClone HKSP is a mouse IgG1 isotype control antibody used in immunological research. Its primary role is to serve as a negative control in antibody-based experiments, particularly in in vivo studies with mice. Isotype controls like HKSP are essential for validating the specificity of experimental antibodies by helping researchers distinguish between specific antibody binding and non-specific background effects. Research ApplicationsThe search results indicate that clone HKSP has been used in various immunological studies. For example, it was employed as a mouse IgG1 isotype control in research examining type I interferon's role in neutrophil swarming and lung immune responses. This demonstrates its utility in ensuring the validity of experimental findings by controlling for non-specific antibody interactions. Limited Citation InformationThe available literature does not provide extensive documentation of key scientific discoveries or breakthrough findings specifically attributed to studies citing clone HKSP. This is consistent with the nature of isotype controls—they are methodological tools that enable rigorous experimental design rather than being the focus of research findings themselves. Their importance lies in supporting the reliability of results obtained with experimental antibodies rather than generating independent discoveries. The clone is designed to have minimal cross-reactivity with mouse cells and tissues, which is crucial for its function as a reliable negative control. Currently, there is no standardized dosing regimen for clone HKSP across different mouse models. The available literature and dosing guides do not provide specific dosing protocols for clone HKSP, and its usage varies significantly depending on several experimental factors. Factors Contributing to Dosing VariabilityThe absence of consistent dosing protocols for clone HKSP stems from multiple variables in experimental design. Mouse models vary widely in their characteristics, including differences in immunocompetence (such as NOD/SCID, NSG, and MISTRG strains), age of the animals, and the specific purpose of the study. This variability makes it difficult to establish universal dosing guidelines that would be applicable across different research contexts. Role as an Isotype ControlIt's important to note that clone HKSP is referenced in scientific literature primarily as an isotype control antibody rather than a therapeutic or functional antibody. As a control reagent, HKSP is used exclusively in experimental design to ensure the validity and specificity of in vivo antibody studies in mice. This control function means that dosing considerations differ fundamentally from those of biologically active compounds or therapeutic antibodies. Implications for ResearchThe lack of standardized dosing regimens complicates data interpretation and comparison across studies. Researchers must independently determine appropriate dosing based on their specific experimental parameters, including the mouse strain selected, the immune function being targeted, and other study-specific variables. This situation highlights the need for more systematic investigation into optimal dosing strategies for clone HKSP across different mouse model systems. References & Citations1. Tzetzo, S. L., Kramer, E. D., Mohammadpour, H., Kim, M., Rosario, S. R., Yu, H., Dolan, M., Oturkar, C. C., Morreale, B., Bogner, P. N., Stablewski, A., Benavides, F., Brackett, C. M., Ebos, J. M., Das, G. M., Opyrchal, M., Nemeth, M. J., Evans, S. S., & Abrams, S. I. (2024). Downregulation of IRF8 in alveolar macrophages by G-CSF promotes metastatic tumor progression. iScience, 109187. https://doi.org/10.1016/j.isci.2024.109187 Technical ProtocolsCertificate of Analysis |
Formats Available
Prod No. | Description |
|---|---|
I-128 | |
I-906 | |
I-907 | |
I-908 | |
I-910 | |
I-104 | |
I-536 | |
I-133 | |
I-103 | |
M1411 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
