Rat IgG1 Isotype Control [Clone GL113] — Purified in vivo GOLD™ Functional Grade
Antibody DetailsProduct DetailsHost Species Rat Recommended Dilution Buffer Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C Working Concentration This isotype control antibody should be used at the same concentration as the primary antibody. RRIDAB_2830523 Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This Rat IgG1 isotype control antibody has been tested against selected species' cells and tissues to assure minimal cross reactivity. Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone GL113 is most commonly used in mice as an in vivo rat IgG1 isotype control antibody. Its primary application is to serve as a negative control in immunological experiments, specifically to distinguish between specific effects of an experimental antibody and nonspecific background effects due to the antibody isotype, Fc interactions, or dosing regimen. Key in vivo uses in mice include:
GL113 is not designed for therapeutic or direct targeting of mouse proteins but is strictly a control reagent. Despite older literature associating the designation GL113 with anti-IL-4 activity, modern commercial sources and standard usage specify it as an isotype control without known mouse reactivity, distinguishing it from functional anti-IL-4 clones such as 11B11. When selecting an in vivo isotype control for mouse studies (e.g., cancer, infection, or immunology models), GL113 is chosen whenever the primary antibody is a rat IgG1, due to its matched subclass and minimal cross-reactive effects. No evidence from current standard references supports a common use of GL113 in vivo as a depleting or neutralizing antibody in mice; its application is nearly universally as an isotype control. Commonly Used Antibodies or Proteins Alongside GL113GL113 is a rat IgG1 isotype control antibody, primarily used as a negative control in experiments—especially in mouse in vivo studies—to help distinguish specific effects of experimental antibodies from non-specific or background effects. While GL113 itself is an isotype control, it is often used alongside other experimental antibodies or proteins designed to target specific biological pathways. However, detailed literature on specific combinations of GL113 with other antibodies/proteins is limited in the provided search results. Antibodies Commonly Used in Experimental Protocols Similar to GL113
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In summary, while GL113 is a standard isotype control, the specific experimental antibodies or proteins used alongside it are dictated by the biological pathway under investigation—most commonly, these include antibodies against cytokines like IL-13 in immunology studies, but direct evidence of co-usage is not provided in the search results. Clone GL113 is a rat IgG1 monoclonal antibody that has been extensively utilized in scientific research, primarily serving as an isotype control antibody in experimental studies. The key findings from its citations in scientific literature reveal its important role in validating experimental results across multiple research domains. Primary Function as Isotype ControlGL113 specifically targets beta-galactosidase but is most commonly employed as a negative control in in vivo mouse experiments. As an isotype control, it provides researchers with a critical baseline to distinguish specific antibody binding from non-specific background effects in immunological studies. This application is essential for ensuring the validity of experimental results when testing other antibodies with biological activity. Applications in Immunological ResearchThe antibody has been referenced across diverse areas of biomedical research. In tumor immunology studies, GL113 served as a control antibody in experiments investigating dendritic cell paralysis reversal using CpG immunostimulatory sequences. The research utilized 5 μg/ml of the control rat IgG1 antibody to validate findings related to antitumor immune responses. GL113 has also been applied in autoimmune disease research, particularly in studies examining inflammatory arthritis and dermatitis in thymectomized, CD25+ cell-depleted mice. In these experiments, both GL113 and PC61 antibodies were used as rat IgG1 standards in ELISA assays, with biotinylated anti-rat IgG1 (clone RG11/39.4) employed for detection purposes. Use in Viral ResearchMore recently, GL113 has been utilized in SARS-CoV-2 research investigating ACE2 as the critical in vivo receptor. Studies administered the antibody at doses of 2 × 500 µg/200 µl as a control to validate findings about viral receptor interactions. The widespread citation of GL113 across multiple research fields underscores its value as a reliable negative control, enabling scientists to confirm that observed effects are due to specific antibody-antigen interactions rather than non-specific binding or other confounding factors. Dosing regimens for clone GL113—a rat IgG1 isotype control—vary substantially depending on the mouse model, experimental application, and administration route. The most widely reported protocol in tumor immunotherapy studies is 1 mg per mouse intraperitoneally (i.p.) every five days for six total doses, spanning about 30 days. Additional dosing approaches have been documented for different study objectives:
Key variables influencing regimen:
Summary Table: GL113 Dosing Across Mouse Models
*Route inferred from standard practice. In summary, 1 mg per mouse intraperitoneally every five days is the most typical regimen, but specific dosing schedules may change according to mouse strain, experimental design, and the intended scientific objective. Where precise regimens are lacking, investigators generally align with these established protocols unless pilot studies indicate alternative pharmacokinetics or efficacy requirements. References & Citations1. Tzetzo, S. L., Kramer, E. D., Mohammadpour, H., Kim, M., Rosario, S. R., Yu, H., Dolan, M., Oturkar, C. C., Morreale, B., Bogner, P. N., Stablewski, A., Benavides, F., Brackett, C. M., Ebos, J. M., Das, G. M., Opyrchal, M., Nemeth, M. J., Evans, S. S., & Abrams, S. I. (2024). Downregulation of IRF8 in alveolar macrophages by G-CSF promotes metastatic tumor progression. iScience, 109187. https://doi.org/10.1016/j.isci.2024.109187 |
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