Rat IgG1 Isotype Control [Clone GL113] — Purified in vivo PLATINUM™ Functional Grade
Rat IgG1 Isotype Control [Clone GL113] — Purified in vivo PLATINUM™ Functional Grade
Product No.: R1379
Clone GL113 Formats AvailableView All Product Type Isotype Control Isotype Rat IgG1 Applications in vivo |
Antibody DetailsProduct DetailsHost Species Rat Recommended Dilution Buffer Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C Working Concentration This isotype control antibody should be used at the same concentration as the primary antibody. RRIDAB_2894259 Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This Rat IgG1 isotype control antibody has been tested against selected species' cells and tissues to assure minimal cross reactivity. Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone GL113 is a rat IgG1 monoclonal antibody most commonly used as an in vivo isotype control in mouse studies. Its primary applications involve serving as a negative control to distinguish the specific effects of experimental antibodies from non-specific background effects caused by the antibody’s Fc region or other non-antigen-specific interactions. Key in vivo applications in mice include:
While GL113's original target is beta-galactosidase, its lack of reactivity in the mouse immune system allows it to function as an ideal isotype control without interfering with most biological processes in vivo. It is not typically used as a functional (neutralizing or depleting) reagent, but solely for control purposes. Some specialized uses may involve testing with encapsulated cells or immunization models to measure non-specific antibody effects, but these are secondary to its main role. GL113 is not to be confused with functional anti-mouse IL-4 antibodies (e.g., clone 11B11), which are used for cytokine neutralization; GL113 specifically serves to control for non-antigen-specific effects in these and similar experiments. While the search results do not provide specific details on other commonly used antibodies or proteins used with GL113 in the literature, they do mention several applications and related antibodies:
Since GL113 is primarily used as a negative control in in vivo mouse studies, other antibodies or proteins used in conjunction with it would typically be those under investigation for their specific biological effects, often in the context of immunological or metabolic studies. For more specific information on proteins or antibodies used alongside GL113, reviewing studies that employ this isotype control might provide additional insights. Clone GL113 is repeatedly cited in scientific literature as a rat IgG1 isotype control antibody. Its principal role is to serve as a negative control in in vivo and in vitro experiments where specific antibody effects need to be distinguished from non-specific background responses. Key findings from cited literature using GL113:
In summary, scientific citations show that clone GL113 is a well-validated rat IgG1 isotype control essential for rigorous immunology, oncology, and infectious disease research, allowing researchers to attribute experimental outcomes to active antibodies rather than isotype background. Dosing regimens of clone GL113 in mouse models vary primarily based on experimental objectives, but the most commonly reported protocol uses 1 mg per mouse administered intraperitoneally every five days for a total of six doses in typical tumor immunotherapy studies. Additional published regimens show variability in dose and schedule:
Key factors influencing particular dosing choices:
For example, studies using GL113 as an isotype control in immunotherapy experiments often apply the higher end of the dosing range (1 mg every five days). In contrast, immunology studies exploring tolerance mechanisms may opt for lower, more frequent doses (100–500 μg every 2–4 days). Published use case: In oral tolerance experiments, mice were injected with GL113 to assess its role as a control antibody; while the specific dose is not always reported, methodology is consistent with standard isotype control regimens. Summary Table: Common GL113 Dosing Regimens in Mouse Models
If your experimental design requires deviation from these standards (e.g., different disease models or antibody pharmacokinetics), adjust dose and frequency based on published controls or pilot dose-finding studies. Always refer to the specific protocol for your mouse strain and disease model to ensure appropriate use. References & Citations1. Tzetzo, S. L., Kramer, E. D., Mohammadpour, H., Kim, M., Rosario, S. R., Yu, H., Dolan, M., Oturkar, C. C., Morreale, B., Bogner, P. N., Stablewski, A., Benavides, F., Brackett, C. M., Ebos, J. M., Das, G. M., Opyrchal, M., Nemeth, M. J., Evans, S. S., & Abrams, S. I. (2024). Downregulation of IRF8 in alveolar macrophages by G-CSF promotes metastatic tumor progression. iScience, 109187. https://doi.org/10.1016/j.isci.2024.109187 |
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