Rat IgG_2a Isotype Control [Clone 1-1] — Purified in vivo GOLD™ Functional Grade

The term clone 1-1 is ambiguous in the context of in vivo mouse studies, as search results do not directly reference a "clone 1-1" antibody or cell line. If you are referring to a specific monoclonal antibody clone (similar to anti-PD-1 clone 4C11 cited in ), or a cell lineage tracing approach (such as clonal labeling/barcoding), the use in in vivo mouse studies will depend on the nature of the clone.

If referring to monoclonal antibodies:

• In vivo mouse studies often use monoclonal antibody clones to block, activate, or deplete target receptors or cell types. For example, the 4C11 clone (anti-mouse PD-1) is a fully murine antibody used in syngeneic mouse tumor models to modulate immune checkpoint signaling. It improves tumor regression and reduces immunogenicity compared to non-murine clones.

If referring to cell lineage tracing/barcoding:

• "Clone" may also relate to individual cell lineages labeled for fate mapping. Clonal analyses in the mouse brain utilize approaches like in utero lentiviral microinjection with unique barcodes or fluorescent labeling vectors, enabling tracking of progenitor cell fate and tissue distribution. These studies identify developmental pathways, migration, and clonal expansion by labeling cells and analyzing progeny after development.

Summary Table: Use of “Clone” in Mouse In Vivo Studies

If you provide the full name, catalog number, or refer to a known monoclonal antibody, a more precise mechanism and experimental protocol can be outlined. Otherwise, both cell lineage tracing and antibody intervention studies use “clones” in in vivo settings to investigate immune, developmental, or regenerative processes in mice.

Recommended Storage Temperature for Sterile Packaged Items

For sterile packaged medical supplies—including items like a "clone 1-1" that appears to be a sterile device or consumable—the standard storage temperature range is between 18ºC and 23ºC (64ºF to 73ºF). This range is widely cited for maintaining the integrity of sterile packaging and preventing premature aging or degradation of the product.

Some guidelines, such as those from the Association of periOperative Registered Nurses (AORN), allow for a slightly broader range of 22ºC to 26ºC (72ºF to 78ºF), but emphasize that temperatures should not exceed these limits to protect sterility.

Other Critical Storage Conditions

• Relative Humidity: Should be maintained between 30% and 60% to prevent moisture-related damage to packaging and contents.
• Storage Environment: Sterile items should be stored in enclosed cabinets or covered carts to avoid exposure to dust and contaminants.
• Airflow: Storage areas should have positive air pressure and adequate air exchanges to minimize airborne contamination.
• Physical Placement: Keep sterile items off the floor, away from walls and ceilings, and never under sinks to avoid water exposure.

Shelf Life Management

• First In, First Out (FIFO): Use older stock first to minimize the risk of aging-related sterility loss.
• Event-Related Shelf Life: Inspect packaging regularly; any breach (tears, punctures, moisture, wrinkles) means the item should be resterilized, regardless of the original expiration date.

Summary Table

Additional Considerations

If your "clone 1-1" is a biological material rather than a standard sterile medical device, storage requirements may differ. Biological samples often require refrigeration or freezing, depending on their nature. However, for most sterile packaged medical devices, the above temperature and humidity ranges are industry standard.

Always refer to the manufacturer’s instructions for your specific product, as some items may have unique requirements. If in doubt, contact the supplier for confirmation.

Commonly Used Antibodies and Proteins Paired with 1-1 in the Literature

While your query specifically mentions "1-1," the search results do not clarify whether this refers to a particular antibody clone, protein, cell line, or another molecular entity. Therefore, a general overview is provided based on the contexts in which antibodies and proteins are most commonly used together in scientific literature.

Typical Antibody-Protein Combinations in Research

• Primary and Secondary Antibodies: In techniques like immunohistochemistry, Western blotting, and ELISA, a primary antibody (specific to the target protein, such as anti-beta actin or anti-GFP) is often paired with a secondary antibody (e.g., anti-mouse IgG or anti-rabbit IgG conjugated to a reporter enzyme or fluorophore) to detect the target protein.
• Monoclonal and Polyclonal Antibodies: Monoclonal antibodies (targeting a single epitope) and polyclonal antibodies (recognizing multiple epitopes on the same antigen) are frequently used, depending on the required specificity and application.
• Isotype Controls: Negative controls often use antibodies of the same species and isotype as the primary antibody but with no specificity for the target protein (e.g., mouse IgG1 as a control for a mouse IgG1 primary antibody).
• Therapeutic Antibody Combinations: In clinical and therapeutic settings, antibodies against immune checkpoints (e.g., anti-PD-1 and anti-CTLA-4) or cytokines (e.g., anti-TNF?) are sometimes used in combination to enhance immune response or treat autoimmune diseases.
• Research Antibodies: Commonly used research antibodies include anti-actin, anti-tubulin, anti-GAPDH (as loading controls), and various cytokine or signaling protein antibodies (e.g., anti-phospho-ERK, anti-p53).

Commonly Used Proteins in Antibody-Based Assays

• Enzymes: Horseradish peroxidase (HRP) and alkaline phosphatase (AP) are standard enzymes conjugated to secondary antibodies for signal detection.
• Fluorescent Proteins: GFP (green fluorescent protein), RFP (red fluorescent protein), and their variants are often co-expressed or detected in cell biology experiments.
• Blocking Proteins: Bovine serum albumin (BSA), non-fat dry milk, or casein are used to block non-specific antibody binding in immunoassays.
• Fusion Proteins: Tags like His-tag, FLAG-tag, or HA-tag are used for protein purification and detection, and their corresponding antibodies (anti-His, anti-FLAG, anti-HA) are standard reagents.
• Therapeutic Proteins: In clinical contexts, proteins like cytokines (IFN-?, IL-2), growth factors (EGF, VEGF), and hormones are often the targets of therapeutic antibodies.

Example Table: Common Antibody-Protein Pairs in Research

Important Considerations

• The choice of antibody or protein depends on the experimental goal—whether for detection, quantification, localization, or functional studies.
• Secondary antibodies are almost always used in conjunction with primary antibodies for signal amplification and detection.
• Fusion tags and their corresponding antibodies are especially common in protein expression and purification workflows.

If "1-1" refers to a specific molecule (e.g., a monoclonal antibody clone, a cell line, or a recombinant protein), please clarify for a more targeted answer.

The search results do not seem to provide specific details about "clone 1-1 citations" in the scientific literature. However, they discuss various aspects related to cloning, such as clone journals and clone pair datasets. Based on the provided information, here are some key findings related to cloning in scientific contexts:

Clone Journals

• Threat to Publishing Integrity: Clone journals pose a significant threat to the integrity of scientific publishing by creating mirror images of reputable journals, potentially leading to the publication of unreviewed manuscripts. These journals may cause authors to lose ownership over their submissions and compromise the validity of research findings.
• Awareness and Consequences: Despite authors being aware of the consequences of publishing in clone journals, many still proceed due to factors like open access at a low cost, which can have serious implications for scientific progress.

Synthetic Clone Pairs

• Dataset Generation: Research into generating synthetic clone pair datasets often involves techniques like grammatical evolution (GE) and large language models (LLMs). The literature review highlights the challenges in finding directly relevant studies for this specific area.

These findings highlight the importance of distinguishing between legitimate and illegitimate forms of cloning in scientific contexts. Clone journals affect the credibility of scientific research, while synthetic clone pairs are used in software engineering and other technical fields for dataset generation.

If you are referring to a specific study or article related to "clone 1-1 citations," additional context or a different query might be needed to provide more precise information.