Rat IgG2a Isotype Control [Clone 1-1] — Purified in vivo GOLD™ Functional Grade
Antibody DetailsProduct DetailsHost Species Rat Recommended Dilution Buffer Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C Working Concentration This isotype control antibody should be used at the same concentration as the primary antibody. RRIDAB_2737530 Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This Rat IgG2a isotype control (anti-Trinitrophenol + KLH) antibody has been tested against selected species' cells and tissues to assure minimal cross reactivity. Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. The term clone 1-1 is ambiguous in the context of in vivo mouse studies, as search results do not directly reference a "clone 1-1" antibody or cell line. If you are referring to a specific monoclonal antibody clone (similar to anti-PD-1 clone 4C11 cited in ), or a cell lineage tracing approach (such as clonal labeling/barcoding), the use in in vivo mouse studies will depend on the nature of the clone. If referring to monoclonal antibodies:
If referring to cell lineage tracing/barcoding:
Summary Table: Use of Clone in Mouse In Vivo Studies
If you provide the full name, catalog number, or refer to a known monoclonal antibody, a more precise mechanism and experimental protocol can be outlined. Otherwise, both cell lineage tracing and antibody intervention studies use clones in in vivo settings to investigate immune, developmental, or regenerative processes in mice. Commonly Used Antibodies and Proteins Paired with 1-1 in the LiteratureWhile your query specifically mentions "1-1," the search results do not clarify whether this refers to a particular antibody clone, protein, cell line, or another molecular entity. Therefore, a general overview is provided based on the contexts in which antibodies and proteins are most commonly used together in scientific literature. Typical Antibody-Protein Combinations in Research
Commonly Used Proteins in Antibody-Based Assays
Example Table: Common Antibody-Protein Pairs in Research
Important Considerations
If "1-1" refers to a specific molecule (e.g., a monoclonal antibody clone, a cell line, or a recombinant protein), please clarify for a more targeted answer. The search results do not seem to provide specific details about "clone 1-1 citations" in the scientific literature. However, they discuss various aspects related to cloning, such as clone journals and clone pair datasets. Based on the provided information, here are some key findings related to cloning in scientific contexts: Clone Journals
Synthetic Clone Pairs
These findings highlight the importance of distinguishing between legitimate and illegitimate forms of cloning in scientific contexts. Clone journals affect the credibility of scientific research, while synthetic clone pairs are used in software engineering and other technical fields for dataset generation. If you are referring to a specific study or article related to "clone 1-1 citations," additional context or a different query might be needed to provide more precise information. Dosing regimens for clone 1-1 (presumed to refer to anti-PD-1 antibodies used in mouse models) typically range from 100–500??g per mouse per injection, administered intraperitoneally (i.p.) every 3–4 days, but can vary based on the specific mouse model, disease context, and experimental objectives. Key details on dosing regimens across models:
Model-Specific Considerations:
Summary Table: Dosing Regimens in Mouse Models
For clone 1-1, if you are referencing a specific anti-PD-1 or PD-L1 clone not detailed in the search results, consult product datasheets or peer-reviewed protocols for confirmation, as regimens may differ slightly by isotype or clone. Always adjust dosing considering model context, immune status, and desired pharmacokinetics. References & Citations1. Gubin, M. et al. (2018) Cell. 175(4):1014–1030.e19 Journal Link 2. Wurster S. et al. (2020) The Journal of Infectious Diseases 222 , 6:1989–994 Journal Link 3. Tzetzo, S. L., Kramer, E. D., Mohammadpour, H., Kim, M., Rosario, S. R., Yu, H., Dolan, M., Oturkar, C. C., Morreale, B., Bogner, P. N., Stablewski, A., Benavides, F., Brackett, C. M., Ebos, J. M., Das, G. M., Opyrchal, M., Nemeth, M. J., Evans, S. S., & Abrams, S. I. (2024). Downregulation of IRF8 in alveolar macrophages by G-CSF promotes metastatic tumor progression. iScience, 109187. https://doi.org/10.1016/j.isci.2024.109187 |
Formats Available
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I-1047 | |
I-1178 | |
R1376 | |
I-1177 | |
R1374 | |
R1367 |
