Rat IgG2b Isotype Control [Clone 1-2] — Purified in vivo GOLD™ Functional Grade

Rat IgG2b Isotype Control [Clone 1-2] — Purified in vivo GOLD™ Functional Grade

Product No.: I-1034

[product_table name="All Top" skus="I-1034"]

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Product. No.I-1034
Clone
1-2
Antibody Type
Isotype Control
Isotype
Rat
Rat IgG2b

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Antibody Details

Product Details

Host Species
Rat
Recommended Dilution Buffer
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Working Concentration
This isotype control antibody should be used at the same concentration as the primary antibody.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
This Rat IgG2b isotype control (anti-Trinitrophenol + KLH) antibody has been tested against selected species' cells and tissues to assure minimal cross reactivity.

Leinco Antibody Advisor

Powered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments.

The term "clone 1-2" is ambiguous. It could refer to an antibody clone (used for targeting specific proteins in vivo), a genetic labeling method (e.g., CLoNe for clonal lineage tracing), or even a specific cell line or engineered variant. Based on your context—"in vivo mouse studies"—the most likely interpretations are:

  1. Antibody Clone for Targeting Mouse Proteins
  2. A Genetic Labeling/Tracing System

There are no direct matches in the search results for “clone 1-2” specifically. However, here's how similar clones (e.g., antibody or clonal tracing systems) are used in mouse in vivo studies:


1. Antibody Clone Usage in In Vivo Mouse Studies

Antibody clones are monoclonal antibodies targeting specific proteins (like PD-1, CD90.2) on mouse cells. Clones such as RMP1-14 (PD-1) or 30-H12 (CD90.2) are commonly named with a number-letter code; e.g., "RMP1-14" or "30-H12".

  • These antibodies are administered to mice to:
    • Block signaling pathways (e.g., immune checkpoint inhibition using anti-PD-1 antibodies).
    • Deplete cell populations (e.g., anti-CD90.2 is used to deplete Thy1.2+ T cells).

The chosen clone impacts the experiment by its species of origin, binding affinity, and functional effects.

Example:

  • Clone 30-H12 (anti-CD90.2): Used for in vivo depletion or identification of Thy1.2-expressing cells in mice.

2. Clonal Labeling Methods (e.g., CLoNe)

If "clone 1-2" refers to clonal lineage analysis, such as the CLoNe (Clonal Labeling of Neural Progenies) technique:

  • CLoNe is used to tag and follow the progeny of single progenitor cells in various mouse tissues, especially neural tissue.
  • Researchers introduce a targeting vector (often using Cre/loxP system) at a controlled concentration to label individual progenitors and their lineages with unique genetic or fluorescent tags.
  • This enables:
    • Tracking of cell lineage and fate.
    • Analysis of clonal relationships and cell behavior in development or disease.

Key features:

  • Multiple clones can be analyzed in one animal.
  • Clones are defined by unique combination of labels, allowing discrimination between different progenitor-derived lineages.

If you meant a clone NOT covered here (like a specific hybridoma, cell line, or genetically engineered cell):

  • Please provide additional details (e.g., target antigen, genetic marker, cell type, or context).
  • With clarification, a more targeted answer can be provided.

Summary:

  • Antibody clones like "30-H12" and "RMP1-14" are injected to modulate or track immune cells in mice.
  • Clonal labeling techniques such as CLoNe allow tracking of cell lineage using unique genetic tags.
  • “Clone 1-2” itself is not found in the referenced literature. Please clarify if you meant a specific antibody, labeling method, or cell line.

Recommended Storage Temperature for Sterile Packaged Clone 1-2

Best Practice for Sterile Medical Supplies

For sterile packaged medical supplies—including items like a "clone 1-2" that are sterilized and intended for medical or laboratory use—the ideal storage temperature is typically between 18°C and 23°C (64°F to 73°F), with a relative humidity between 30% and 60%. These conditions help maintain the integrity of the packaging and prolong shelf life.

Considerations Based on Packaging Type and Manufacturer Instructions

  • Always follow the manufacturer’s instructions: If the packaging or product label specifies a different temperature or humidity, those instructions take precedence over general guidelines.
  • Environmental control: The storage area should be clean, well-ventilated, and protected from contamination, moisture, dust, temperature extremes, and humidity extremes. Avoid storing supplies in areas where environmental conditions fluctuate.
  • Documentation and compliance: While most guidelines recommend monitoring and recording temperature and humidity daily, actual documentation may only be required if specified by the manufacturer or regulatory body.

Additional Guidelines

  • The Sterilization Packaging Manufacturers Council (SPMC) suggests a broader range of 10°C to 27°C (50°F to 80°F) for packaging materials in general, but medical sterile supplies should ideally stay within the narrower, controlled range above for optimal sterility maintenance.
  • For commercially sterilized products, breakout rooms adjacent to sterile storage areas are recommended to safely transition products from shipping containers to storage.
  • Event-related shelf life: Regardless of time, any package with compromised integrity (tears, punctures, moisture, etc.) should be considered non-sterile and reprocessed.

Summary Table

ConditionRecommended ValueSource
Temperature18°C – 23°C (64°F – 73°F)
Relative Humidity30% – 60%
Follow ManufacturerAlways check product label/instructions
Environmental ControlClean, dry, dust-free, ventilated

Conclusion

Store your sterile packaged clone 1-2 at 18°C to 23°C with 30% to 60% relative humidity, in a clean, controlled environment, unless the manufacturer specifies otherwise. Always prioritize packaging integrity and follow any additional instructions provided with the product.

Commonly Used Antibodies and Proteins in Conjunction with 1-2

When researchers refer to “1-2,” they likely mean monoclonal antibodies (mAbs) or peptide–antibody complexes—especially given the context of antibody research, structure, and cross-reactivity. Here, we outline the types of antibodies and proteins most frequently referenced alongside “1-2” in the literature, based on recent structural, immunological, and therapeutic studies.

Antibodies and Proteins in Antibody–Peptide Recognition

  • Monoclonal Antibodies (e.g., H1-74, HCV1, 10E8, 2F5, 4E10): These are commonly studied for their ability to bind antigens and peptides. For example, the H1-74 mAb interacts with multiple influenza hemagglutinin (HA) peptides and synthetic variants, illustrating both specificity and cross-reactivity depending on key amino acid residues in the complementarity-determining region (CDR). HCV1 targets a linear epitope in HCV E2, while 10E8, 2F5, and 4E10 are HIV antibodies that bind to the membrane-proximal external region (MPER) of Env, often influenced by membrane lipids.
  • Polyclonal Antibodies: Used in diagnostic and therapeutic contexts, such as anti-toxins for botulism and Rh(D) immunoglobulins.
  • Recombinant Antibodies: Used in research, diagnostics, and therapeutics. Examples include MAbs to CD markers (CD20, CD34), tumor markers (EGFR, B-Raf), and checkpoint inhibitors (anti-PD-1, e.g., Nivolumab, Pembrolizumab).
  • Secondary Antibodies (Conjugated to Enzymes or Fluorescent Dyes): Commonly used in assays like ELISA, Western blot, and immunohistochemistry. Conjugates include horseradish peroxidase, alkaline phosphatase, fluorescent dyes (Alexa Fluor, DyLight, Cy, etc.), and biotin.

Proteins and Peptides Used in Conjunction with Antibodies

  • Fusion Proteins and Artificial Antigens: Engineered for immunization, diagnostics, and epitope mapping. These are often synthetic peptides or recombinant proteins that mimic natural epitopes.
  • Enzyme Conjugates (e.g., Horseradish Peroxidase, Alkaline Phosphatase): Frequently used with secondary antibodies in detection systems.
  • Membrane Proteins and Lipids: In the case of antibodies like 10E8, 2F5, and 4E10, membrane lipids are critical co-factors for HIV neutralizing activity.
  • Streptavidin/Biotin Systems: Used to amplify detection signals in immunoassays.
  • IgG, F(ab’)?, and Fab Fragments: Commonly used to study antigen binding without effector functions or for specific detection in assays.

Application Areas

  • Structural Biology: Studies on antibody–peptide complexes, such as those cited above, focus on how antibodies recognize and bind peptides, often revealing mechanisms of cross-reactivity and conformational variability.
  • Diagnostics: Antibodies are used in ELISA, IHC, flow cytometry, and Western blotting, often in combination with secondary antibodies and enzyme conjugates.
  • Therapeutics: Monoclonal antibodies, antibody–drug conjugates, and polyclonal preparations are used for treating cancers, autoimmune diseases, and infectious diseases.

Summary Table

TypeExamples/ApplicationsNotes
Monoclonal AntibodiesH1-74, HCV1, 10E8, 2F5, 4E10, anti-CD20, anti-EGFR, anti-PD-1Used for structure-function studies, diagnostics, therapeutics
Polyclonal AntibodiesAnti-toxins, Rh(D) IgUsed in prophylaxis and therapy
Secondary AntibodiesHRP, AP, Alexa Fluor, DyLight, Cy, BiotinUsed in immunoassays for signal detection
Fusion/Artificial ProteinsEngineered peptides, recombinant antigensFor immunization and epitope mapping
Enzyme ConjugatesHRP, APSignal amplification in assays
Membrane Proteins/LipidsInvolved in MPER antibody activityCritical for certain neutralizing antibodies
Biotin/StreptavidinUsed in detection systemsSignal amplification

Key Points

  • Monoclonal antibodies and engineered peptides are central to studies of antibody–antigen interactions, including cross-reactivity and conformational binding.
  • Secondary antibodies conjugated to enzymes or fluorophores are ubiquitous in immunoassays.
  • Membrane proteins and lipids can be critical for the activity of certain antibodies, especially in viral contexts.
  • Recombinant and polyclonal antibodies are widely used in diagnostics and therapeutics.

If “1-2” refers to a specific antibody pair or a published set of antibodies, please clarify for a more targeted answer. The above covers the most common and relevant proteins and antibodies referenced in antibody research literature.

Key findings from two representative scientific citations about "clone" focus on both laboratory cloning techniques and issues related to "cloned journals":

  • In-house Production and Efficiency of Cloning Vectors:
    A study demonstrated that a self-made pJET1.2/blunt cloning vector enables efficient, low-cost, and high-fidelity cloning of DNA fragments of varying sizes, comparable to commercial alternatives. The process is straightforward, does not require phosphorylation before ligation, and significantly simplifies DNA cloning workflows in molecular biology labs.

  • Authors' Motivations and Awareness in Cloned Journal Publications:
    Research into "cloned journals" (unauthorized copies of reputable journals) found that 79% of surveyed authors agreed with multiple reasons for publishing in these journals, the most common being low-cost open access (86% agreement). Strikingly, a similar proportion were aware of the negative consequences, including hampering scientific progress (69% agreement), yet many still pursued such publications for academic credit.

These findings highlight major issues in both practical cloning methods and the ethics of journal cloning in scientific communication.

References & Citations

1.) Shin, Haina et al. (2018) J Virol. 92(7): e00038-18. PubMed
2.) Hawman DW, et al. (2021) Microorganisms 9(2):279 Journal Link

Formats Available

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.