Rat IgG2b Isotype Control [Clone 1-2] — Purified in vivo GOLD™ Functional Grade

Rat IgG2b Isotype Control [Clone 1-2] — Purified in vivo GOLD™ Functional Grade

Product No.: I-1034

[product_table name="All Top" skus="I-1034"]

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Product. No.I-1034
Clone
1-2
Antibody Type
Isotype Control
Isotype
Rat
Rat IgG2b

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Antibody Details

Product Details

Host Species
Rat
Recommended Dilution Buffer
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Working Concentration
This isotype control antibody should be used at the same concentration as the primary antibody.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
This Rat IgG2b isotype control (anti-Trinitrophenol + KLH) antibody has been tested against selected species' cells and tissues to assure minimal cross reactivity.

Leinco Antibody Advisor

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Clone 1-2 most commonly refers to a rat IgG2b isotype control antibody used in mouse studies as a negative control for in vivo experiments involving rat monoclonal antibodies. Its primary applications are not to induce a biological effect, but to serve as a control for nonspecific binding and immune background when administering monoclonal antibodies therapeutically or experimentally in mice.

Essential context and supporting details:

  • Purpose: In vivo, clone 1-2 is used as a functional-grade isotype control—a class-matched, non-specific antibody to compare with specific monoclonal antibodies (such as anti-cytokine or anti-surface marker antibodies), providing a baseline for interpreting biological effects and immune reactions. This helps distinguish the actual effect of the targeted therapeutic antibody from background immune modulation caused by the presence of any foreign IgG in the system.
  • Formulation: The antibody is typically administered purified and at doses matched to the experimental monoclonal antibody, often via intravenous, intraperitoneal, or subcutaneous injection in mice.
  • Use cases:
    • As a negative control in models where investigators want to block or deplete certain immune factors or cell populations (e.g., when using functional antibodies like anti-CD4, anti-CD8, anti-IFNγ, etc.).
    • In studies of autoimmunity, infectious disease, or cancer, wherever in vivo blocking or depletion strategies are used.
  • Optimizing dose and regimen: The dosing should mirror and control for the experimental monoclonal antibody, often referencing supplier protocols or published optimization regimens. Researchers may titrate dose or frequency for specific experimental needs.

Limitations:

  • If you meant "clone 1-2" as a reference to something other than a rat IgG2b isotype control (for example, a cell line, genetically engineered cell, or a specific gene clone), clarification is needed. The above applications are for the isotype control antibody, which is by far the most common usage in published in vivo mouse literature.

No direct information for unique biological or therapeutic targeting by clone 1-2 was found; it is not itself used to deplete cell types or neutralize cytokines, but solely as a negative control for antibody-based in vivo mouse studies.

Commonly used antibodies and proteins in the literature, when used together or in multiplexed experimental contexts, include CD14, CD20, CD34, isocitrate dehydrogenase, B-Raf, epidermal growth factor receptor (EGFR), and melanoma glycoprotein B among many others. These antibodies target well-characterized surface markers or intracellular proteins, and are frequently applied in research, diagnostics, and therapeutics across varied fields.

Frequently paired or multiplexed antibodies/proteins include:

  • Surface marker antibodies: CD14, CD20, CD34 are employed together for cell type characterization, flow cytometry, and immunophenotyping (e.g., hematopoietic, immune lineage studies).
  • Signal transduction proteins/antibodies: B-Raf, isocitrate dehydrogenase, and EGFR antibodies are often used together to profile signaling pathways, assess mutations, or study oncogenic processes in diagnostics and research.
  • Diagnostic protein antibodies: Melanoma glycoprotein B and antibodies for viral proteins (e.g., herpes simplex virus) are co-utilized in ELISA and immunohistochemistry panels designed for disease profiling.
  • Therapeutic monoclonal antibodies (MAbs):
    • Rituximab (CD20 targeting), Ocrelizumab (CD20), Infliximab (TNF), Nivolumab (PD-1), Panitumumab (EGFR), Daclizumab (CD25), and antibody-drug conjugates are often referenced together in the context of immunotherapy and clinical trials.
    • Such therapeutics may be compared or used sequentially/combinatorially depending on disease and patient needs.

Additional commonly referenced proteins/antibodies:

  • Secondary antibodies: Used in conjunction with a wide array of primary antibodies for detection, including anti-rabbit IgG, anti-mouse IgG, etc. Secondary antibodies may be conjugated to fluorescent or enzymatic labels for multiplex or multicolor experiments.
  • Proteomic experiments: Panels often incorporate antibodies against total protein markers (e.g., β-actin, GAPDH) and phosphorylation-specific antibodies for signaling studies.
  • Vaccine development: Polyclonal antibodies (PAbs) for infectious agent profiling and monitoring immune response, as seen in MMR, DiTePePolHiB, and HPV vaccines.

Experimental multiplexing frequently involves combining antibodies:

  • Against cell surface and intracellular markers
  • Against post-translationally modified epitopes (e.g., phosphorylated proteins)
  • Using species-specific antibody panels (e.g., mouse and rabbit antibodies detected with species-appropriate secondaries)

In summary, CD markers, tyrosine kinases (e.g., B-Raf, EGFR), and checkpoint/therapeutic antibodies are among the most commonly used with others in the literature for simultaneous detection, phenotyping, and clinical interventions.

Key findings from scientific literature about "clone 1-2 citations" fall into two main areas: cloning technologies and the phenomenon of cloned (or duplicate) citations/journals.

1. Cloning Technologies:

  • High Efficiency of Self-Made Vectors: The in-house produced pJET1.2/blunt cloning vector demonstrates 100% cloning efficiency for small (538 bp) and larger DNA fragments (up to 2.3 kb), equivalent to commercial vectors.
  • The protocol for vector preparation is straightforward and cost-effective, requiring no phosphorylation of DNA fragments prior to ligation.
  • This vector supports routine, rapid DNA cloning and screening in molecular biology labs, particularly for blunt-ended DNA fragments.

2. Cloned Citations & Journals:

  • Multiple Causes for Publishing in Cloned Journals: Researchers agree (average 79%) there are diverse motivations for submitting to cloned journals, with the leading cause being open access at low cost (86% agreement), and the lowest being academic promotion (71% agreement).
  • Awareness of Negative Consequences: Authors are often aware (average 79%) of the negative impacts, such as wrongfully motivating others (85%) or producing misleading scientific conclusions (69%).
  • Despite this, many continue to seek academic credit from these publications, indicating systematic issues in the research ecosystem.

These findings highlight both advances in molecular cloning techniques and ongoing ethical/practical challenges associated with duplicated or cloned citations and journals.

Dosing regimens for clone 1-2 antibodies in mouse models can vary substantially based on the mouse strain, disease context, and specific experimental goals, and there is no single universal schedule. Published literature and supplier protocols remain the most authoritative sources for precise dosage details for this clone.

Key factors influencing dosing regimens for clone 1-2 include:

  • Mouse model and strain: Immunodeficient, transgenic, or wild-type mice may require different dosing volumes and frequencies to achieve desired outcomes.
  • Disease or experimental context: For example, oncology, immunology, or infection models may use different regimens based on disease burden or study endpoints.
  • Endpoint objectives: The desired pharmacodynamic effect, such as immune cell depletion, cytokine modulation, or receptor blockade, often determines both dose and dosing frequency.

General principles (based on in vivo antibody dosing guides):

  • Typical antibody doses (not specific to clone 1-2) in mouse models range from 100–500 μg per mouse per dose, usually administered by intraperitoneal injection.
  • Dosing frequency often ranges from every 3–7 days, with some protocols utilizing repeated administration over multiple weeks depending on antibody half-life and disease kinetics.
  • Optimization: Researchers are frequently advised to conduct pilot titration experiments or consult literature for protocol adjustments, as inadequate dosing may lead to subtherapeutic or toxic effects, while excessive doses can confound immunogenicity assessment.
  • Isotype and background controls: The same dosing guidelines typically apply for isotype control clones (such as clone 1-2, if used as IgG2b isotype control), but confirm in the context of each specific experimental system.

For precise application of clone 1-2, consult targeted supplier guidelines or primary publications where this clone has been used. If such data is unavailable or ambiguous, start with general recommendations for rat IgG2b isotype antibodies and adjust based on observed responses, always including necessary controls.

References & Citations

1.) Shin, Haina et al. (2018) J Virol. 92(7): e00038-18. PubMed
2.) Hawman DW, et al. (2021) Microorganisms 9(2):279 Journal Link

Formats Available

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.