Bone morphogenetic protein receptor type II or BMPR2 is a serine/threonine receptor kinase. It binds Bone morphogenetic proteins, members of the TGF beta superfamily of ligands. BMPs are involved in host of cellular functions including osteogenesis, cell growth and cell differentiation. Signaling in the BMP pathway begins with the binding of a BMP to the the type II receptor. This causes the recruitment of a BMP type I receptor, which it phosphorylates. The Type I receptor phosphorylates an R-SMAD a transcriptional regulator. Unlike the TGFβ type II receptor, which has a high affinity for TGF-β1, BMPR2 does not have a high affinity for BMP-2, BMP-7 and BMP-4, unless it is co-expressed with a type I BMP receptor. In TGF beta signaling all of the receptors exist in homodimers before ligand binding. In the case of BMP receptors only a small fraction of the receptors exist in homomeric forms before ligand binding. Once a ligand has bound to a receptor, the amount of homomeric receptor oligomers increase, suggesting that the equilibrium shifts towards the homodimeric form. The low affinity for ligands suggests that BMPR2 may differ in the from other type II TGF beta receptors in that the ligand may bind the type I receptor first. Human and mouse BMPR-II are highly conserved sharing 97% sequence identity.
The molecular weight of Recombinant Human BMPR-II is Mr 65-75 kDa.
Predicted Molecular Mass
41.5
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
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Recombinant Human BMPR-II (Bone Morphogenetic Protein Receptor Type II) is used in research to study and manipulate the BMP signaling pathway, which is critical for vascular biology, bone development, and diseases such as pulmonary arterial hypertension (PAH). Using recombinant BMPR-II allows precise control over receptor levels and activity in experimental systems, enabling detailed investigation of its biological roles and therapeutic potential.
Key reasons to use recombinant human BMPR-II in research applications:
Modeling and Restoring BMPR-II Function: Recombinant BMPR-II can be used to restore or enhance BMPR-II signaling in cell and animal models with BMPR2 mutations or deficiencies, which is relevant for studying diseases like PAH where BMPR-II loss is a central pathogenic mechanism.
Mechanistic Studies: It enables dissection of downstream signaling events, ligand-receptor interactions, and cellular responses to BMP ligands (such as BMP9 and BMP10), which are important for understanding endothelial function, vascular integrity, and apoptosis.
Therapeutic Screening: Recombinant BMPR-II is valuable for screening small molecules, antibodies, or other biologics that modulate BMP signaling, supporting drug discovery efforts targeting this pathway.
Biochemical and Structural Analysis: Purified recombinant BMPR-II is essential for in vitro binding assays, structural biology (e.g., crystallography or cryo-EM), and mapping interaction sites with BMP ligands or other signaling partners.
Additional context:
In PAH research, restoring BMPR-II function or enhancing its signaling (for example, by supplementing with recombinant BMP ligands) has shown promise in reversing disease phenotypes in preclinical models.
Recombinant BMPR-II can be used as a standard or control in ELISA, Western blot, or other immunoassays to quantify receptor expression or activity.
In summary, recombinant human BMPR-II is a versatile tool for elucidating BMP pathway biology, modeling disease, and developing targeted therapies, especially in contexts where BMPR-II function is compromised.
Yes, you can use Recombinant Human BMPR-II as a standard for quantification or calibration in your ELISA assays, provided that the recombinant protein is compatible with the detection antibodies and assay conditions of your specific ELISA kit.
Key Considerations:
Immunological Compatibility: The recombinant BMPR-II must be recognized by both the capture and detection antibodies used in your ELISA. Most commercial ELISA kits are designed to detect both natural and recombinant forms of the target protein, but you should verify this in the kit’s manual or product specifications.
Protein Formulation: Recombinant proteins are often supplied with carrier proteins (e.g., BSA) or stabilizers. If present, these additives may interfere with the assay or affect the standard curve. For best results, use a recombinant standard formulated without interfering substances, or ensure that your assay diluent and buffers are compatible.
Purity and Concentration: The recombinant standard should be highly purified and accurately quantified. The concentration must be known precisely to generate a reliable standard curve.
Matrix Effects: The recombinant protein should be diluted in a matrix that closely matches your sample matrix (e.g., assay buffer, serum, or plasma) to minimize matrix effects and ensure accurate quantification.
Validation: It is good practice to validate the use of your recombinant standard by running a standard curve and confirming linearity, sensitivity, and recovery within the expected range of your assay.
Example from Literature:
Recombinant human BMP-2 is commonly used as a standard in BMP-2 ELISA assays, and similar principles apply to BMPR-II.
Some manufacturers recommend using their own recombinant protein as a standard for optimal performance.
Recommendation:
Check your ELISA kit’s manual for specific guidance on standard selection. If the kit does not specify, contact the manufacturer to confirm compatibility. If validated, recombinant Human BMPR-II is a suitable and reliable standard for ELISA quantification.
Recombinant Human BMPR-II has been validated for several key applications in published research, primarily in the context of studying BMP signaling, pulmonary arterial hypertension (PAH), and protein-protein interactions.
Validated Applications in Published Research:
Cell Signaling Studies: Recombinant human BMPR-II is widely used to investigate BMP (Bone Morphogenetic Protein) signaling pathways, particularly in endothelial and smooth muscle cells. It is essential for dissecting the mechanisms of BMPR-II-mediated signal transduction, including Smad-dependent and Smad-independent pathways.
Pulmonary Arterial Hypertension (PAH) Research: Recombinant BMPR-II has been used in in vitro and in vivo models to study the restoration of BMPR-II signaling in PAH. This includes:
Assessing the effects of BMPR-II supplementation or gene therapy on endothelial function and disease progression.
Evaluating the impact of exogenous BMP ligands (such as BMP9) on BMPR-II expression and downstream signaling in patient-derived cells and animal models.
Protein-Protein Interaction Analyses: Recombinant BMPR-II is employed in binding assays to characterize interactions with BMP ligands (e.g., BMP-2, BMP-4, BMP-7, BMP-9) and type I receptors. These studies help elucidate receptor-ligand specificity and affinity, as well as the structural basis of receptor activation.
Immunological and Cell Biology Studies: Recombinant BMPR-II is used as a standard or control in ELISA, Western blot, and immunoprecipitation assays to quantify or detect BMPR-II protein levels in various biological samples.
Therapeutic Development: Recombinant BMPR-II serves as a tool for screening and validating small molecules or biologics that modulate BMPR-II activity, with the goal of developing therapies for diseases such as PAH and certain cancers.
Additional Context:
Recombinant BMPR-II is often used in combination with other BMP pathway components (e.g., type I receptors, BMP ligands) to reconstitute signaling complexes in vitro.
Functional studies may involve overexpression or supplementation of recombinant BMPR-II in cell lines or animal models to assess phenotypic rescue or pathway activation.
Summary Table:
Application Area
Example Use Case
Cell signaling studies
Dissecting BMP/Smad signaling in endothelial cells
PAH research
Restoring BMPR-II function in patient-derived cells and animal models
Protein-protein interaction
Binding assays with BMP ligands and type I receptors
Immunological/cell biology assays
ELISA, Western blot, immunoprecipitation for BMPR-II quantification/detection
Therapeutic development
Screening modulators of BMPR-II for drug discovery
If you need protocol-specific details or references for a particular application, please specify the experimental context.
To reconstitute and prepare Recombinant Human BMPR-II protein for cell culture experiments, add sterile water to the lyophilized powder to achieve a stock concentration of 0.5 mg/mL or within the range of 0.1–1.0 mg/mL, depending on your experimental requirements.
Essential steps and considerations:
Centrifuge the vial briefly before opening to ensure all powder is at the bottom.
Add sterile, deionized water to the vial. For most BMPR-II preparations, a concentration between 0.1–1.0 mg/mL is recommended.
Gently mix by pipetting up and down or by gentle vortexing. Avoid vigorous agitation to prevent protein denaturation.
If recommended by the supplier or for enhanced stability, add 5–50% glycerol (final concentration) to the solution.
Do not use PBS or cell culture media for initial reconstitution, as salts and other components may precipitate or denature the protein unless the formulation specifically allows it.
Aliquot the stock solution into small volumes to avoid repeated freeze-thaw cycles.
Storage:
Lyophilized protein: Store at –20°C to –80°C for up to 12 months.
Reconstituted protein: Store at 4–8°C for short-term use (2–7 days), or at –20°C for longer-term storage (up to 3 months).
For cell culture applications, dilute the stock solution into your culture medium immediately before use. If required, include a carrier protein such as tissue culture grade BSA or heat-inactivated FCS at 0.1% to minimize adsorption and loss.
Additional notes:
Always consult the specific product datasheet for formulation details and recommended diluents, as some BMPR-II proteins may be supplied with stabilizers (e.g., trehalose, mannitol) or in PBS, which may affect reconstitution protocols.
Confirm the protein’s bioactivity if required for your assay, as some preparations may not be validated for functional activity.
Use endotoxin-free reagents and sterile technique throughout to ensure suitability for cell culture.
Summary protocol:
Briefly centrifuge vial.
Add sterile water to achieve 0.1–1.0 mg/mL.
Mix gently.
Aliquot and store appropriately.
Dilute into cell culture medium with carrier protein if needed.
This protocol ensures optimal solubility, stability, and activity of recombinant BMPR-II for cell culture experiments.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
