Recombinant Human IGF-I (Previously QED Part No.: 20802P)

IGFs (Insulin-like growth factors) are polypeptide growth factors that prompt cell growth and survival. Produced primarily by the liver, they affect muscle, bone, and cartilage tissues. As part of the insulin gene family, IGFs are structurally similar to insulin but possess greater growth-promoting activity. IGF-I and IGF-II are regulated by growth hormone and placental lactogen, respectively. They signal via the tyrosine kinase type I receptor and are created from inactive precursor proteins through proteolytic processing. This process involves the removal of N-terminal and C-terminal propeptide regions, transforming them into their mature, active forms. The IGFs' ability to stimulate proliferation makes them crucial for various developmental and regenerative processes in the body. The IGF-I protein associates with IGF binding proteins thereby increasing its plasma half‑life and modulating its interactions with receptors.

A cell proliferation assay with serum-free human MCF-7 cells was used to determine the biological activity. The Specific Activity was found to be greater than 5.0×10₅ IU/mg, which corresponds to an effective concentration of less than 2 ng/ml.

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Lyophilized

The predicted molecular weight of Recombinant Human IGF-I is Mr 7.6 kDa.

7.6

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.

To make a solution of IGF-1, you should first mix the freeze-dried powder with sterile water that has a resistance of 18 MΩ-cm. The concentration of this initial mixture should be at least 100 µg/ml. Once you have this concentrated solution, you can dilute it further with other water-based solutions.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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