Interleukin 12 receptor, beta 1 (IL12RB1), also known as CD212, is a type I transmembrane protein that belongs to the gp130/G-CSF receptor family. It is a common subunit of both the IL-12 and IL-23 receptor complexes which play roles in T cell mediated inflammatory reactions (1). IL12RB1 binds IL-12 with low affinity and is involved in IL-12 transduction (2). In association with IL23R, it forms the IL-23 receptor complex which functions in IL-23 signal transduction probably through activation of the Jak-STAT signaling cascade (3). IL12RB1 is expressed on activated T cells, B cells, NK cells, macrophages, and microglia. Defects in IL12RB1 are generally associated with selective susceptibility to weakly pathogenic mycobacteria and Salmonella species (4). Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
The predicted molecular weight of Recombinant Human IL-12 Rβ1 is Mr 84.5 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 110 kDa.
Predicted Molecular Mass
84.5
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
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Using Recombinant Human IL-12 Rβ1 in research is essential for studying the biology and signaling of the IL-12 receptor, dissecting immune pathways, and developing or screening immunotherapies that target or utilize the IL-12 axis.
Key reasons to use Recombinant Human IL-12 Rβ1 in research applications:
Receptor-Ligand Interaction Studies: IL-12 Rβ1 is a critical subunit of the functional IL-12 receptor complex. Recombinant IL-12 Rβ1 enables precise in vitro analysis of how IL-12 (and related cytokines like IL-23) bind and activate their receptor, allowing for detailed mapping of binding sites, affinity measurements, and structure-function relationships.
Signal Transduction Research: By providing a defined, purified receptor subunit, researchers can reconstitute IL-12 signaling pathways in cell-based or cell-free systems. This is crucial for dissecting downstream events such as STAT4 activation, IFN-γ induction, and Th1 differentiation, which are central to cellular immunity and anti-tumor responses.
Immunological Assays and Screening: Recombinant IL-12 Rβ1 can be used in ELISA, flow cytometry, or surface plasmon resonance assays to detect or quantify IL-12, screen for neutralizing antibodies, or identify small molecules that modulate IL-12 signaling. This is valuable for drug discovery and immunotherapy development.
Functional Characterization of Mutations: Mutations in IL-12 Rβ1 are linked to immunodeficiencies. Recombinant protein allows for functional assays to characterize the impact of specific mutations on ligand binding and signaling, aiding in both basic research and clinical diagnostics.
Receptor-Binding Research: The availability of recombinant IL-12 Rβ1 with confirmed purity and activity ensures reproducibility and reliability in experiments focused on receptor-ligand interactions, competitive binding, and receptor cross-talk with other cytokines.
Additional context:
IL-12 signaling is pivotal for Th1 immune responses, NK cell activation, and anti-tumor immunity.
Recombinant receptor subunits are indispensable for mechanistic studies, therapeutic antibody development, and validation of new immunomodulatory agents.
In summary, Recombinant Human IL-12 Rβ1 is a versatile tool for immunology, oncology, and translational research, enabling precise dissection of IL-12-mediated pathways and supporting the development of targeted therapies.
Yes, you can use Recombinant Human IL-12 Rβ1 as a standard for quantification or calibration in ELISA assays, provided that your assay is designed to detect IL-12 Rβ1 and the recombinant protein is compatible with your detection system.
Key Points:
Purpose as a Standard: Recombinant proteins like Recombinant Human IL-12 Rβ1 are commonly used as standards in ELISA assays to generate a standard curve, which allows for the quantification of IL-12 Rβ1 in unknown samples.
Carrier Protein Consideration: If the recombinant protein is supplied with BSA (bovine serum albumin), it is generally recommended for use in cell culture or as an ELISA standard. If it is carrier-free, it is suitable for applications where BSA might interfere (e.g., certain functional assays or when BSA could cause background issues).
Assay Compatibility: Ensure that your ELISA kit or protocol is validated for use with recombinant IL-12 Rβ1 and that there is no cross-reactivity or interference from other components.
Reconstitution and Handling: Follow the manufacturer's instructions for reconstitution and storage to maintain protein stability and activity.
References:
R&D Systems: Recommends using recombinant proteins with BSA for ELISA standards.
Upingbio ELISA Kit: Uses recombinant IL-12 Rβ1 as a calibrator in their ELISA kit.
In summary, Recombinant Human IL-12 Rβ1 is suitable for use as a standard in ELISA assays for the quantification of IL-12 Rβ1, as long as it is compatible with your specific assay conditions and detection method.
Recombinant Human IL-12 Receptor Subunit Beta-1 (IL-12Rβ1) has been validated for several key applications in published research, primarily centered around immunological and receptor-binding studies. Based on the available literature and product documentation, the main validated applications include:
Immunological Research: IL-12Rβ1 is used to study the structure and function of the IL-12 receptor complex, including its role in signal transduction and immune cell activation. It is essential for understanding the mechanisms of IL-12 and IL-23 signaling, which are critical for T cell and NK cell responses.
Receptor-Binding Assays: The protein is validated for use in receptor-binding studies to investigate the interaction between IL-12 and its receptor subunits. This includes assessing the binding affinity and specificity of IL-12 to IL-12Rβ1, which is crucial for understanding the molecular basis of IL-12 signaling.
Signal Transduction Studies: IL-12Rβ1 is used to study the downstream signaling pathways activated by IL-12, such as the activation of Janus kinases (JAKs) and phosphorylation of STAT proteins. These studies help elucidate the cellular responses to IL-12 and the regulation of immune functions.
Functional Assays: The protein is used in functional assays to evaluate the biological activity of IL-12 and its receptor subunits. This includes assessing the ability of IL-12Rβ1 to mediate the effects of IL-12 on immune cell proliferation, differentiation, and cytokine production.
Therapeutic Development: IL-12Rβ1 is used in the development and testing of therapeutic agents targeting the IL-12/IL-23 pathway. This includes the evaluation of antibodies and small molecules that modulate the activity of IL-12Rβ1, which is relevant for the treatment of autoimmune diseases and cancer.
Structural Studies: The protein is used in structural biology studies to determine the three-dimensional structure of the IL-12 receptor complex, which is important for understanding the molecular mechanisms of receptor-ligand interactions and for the design of novel therapeutics.
These applications highlight the importance of Recombinant Human IL-12 Rβ1 in both basic and applied research, contributing to our understanding of immune regulation and the development of new therapeutic strategies.
To reconstitute and prepare Recombinant Human IL-12 Rβ1 protein for cell culture experiments, follow these best-practice steps based on current protocols for this protein:
Reconstitution Buffer: Use sterile PBS (phosphate-buffered saline) as the primary reconstitution buffer. Some protocols specify pH 7.2–7.4.
Concentration: Reconstitute the lyophilized protein to a final concentration typically between 0.1–1.0 mg/mL. Some sources recommend specific concentrations such as 200–250 μg/mL in PBS.
Mixing: Gently swirl or tap the vial to dissolve the protein. Do not vortex, as this can denature or aggregate the protein.
Optional Stabilizers: For extended storage or to prevent adsorption, you may add carrier proteins such as 0.1% endotoxin-free recombinant human serum albumin (HSA) or bovine serum albumin (BSA). Some protocols also recommend adding 5–50% glycerol for cryoprotection if you plan to freeze aliquots.
Aliquoting: After reconstitution, aliquot the solution into single-use volumes to avoid repeated freeze-thaw cycles, which can degrade the protein.
Sterility: Ensure all solutions and materials are sterile to prevent contamination in cell culture applications.
Example Protocol:
Briefly centrifuge the vial to collect the lyophilized powder at the bottom.
Add sterile PBS (pH 7.2–7.4) to achieve your desired concentration (e.g., 0.2 mg/mL or as specified in your experimental design).
Gently swirl or tap the vial until the protein is fully dissolved. Do not vortex.
If desired, add 0.1% HSA/BSA or 5–50% glycerol for stabilization.
Aliquot and store at 2–8 °C for short-term use (up to 1 month) or at –20 °C to –70 °C for long-term storage.
Avoid repeated freeze-thaw cycles.
Notes:
Always consult the specific Certificate of Analysis (CoA) or datasheet for your protein lot, as formulation and recommended conditions may vary.
For cell culture, ensure the final buffer and additives are compatible with your assay and cell type.
Summary Table:
Step
Recommended Practice
Buffer
Sterile PBS, pH 7.2–7.4
Concentration
0.1–1.0 mg/mL (commonly 200–250 μg/mL)
Mixing
Gentle swirling/tapping, no vortexing
Stabilizer (optional)
0.1% HSA/BSA or 5–50% glycerol
Aliquoting
Single-use aliquots
Storage
2–8 °C (≤1 month), –20 °C to –70 °C (long-term)
Freeze-thaw cycles
Avoid repeated cycles
These guidelines will help ensure the bioactivity and stability of IL-12 Rβ1 for your cell culture experiments.
References & Citations
1. Becker, C. et al. (2005) Inflamm. Bowel. Dis. 11:755
2. Ling, P. et al. (1995) J. Immunol.154:116
3. Parham, C. et al. (2002) J. Immunol. 168:5699
4. Ersoy, F. et al. (2007) Pediatr. Infect. Dis. J. 26:366
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
