Recombinant Human Nanog

Nanog, a homeobox transcription factor¹, plays a key role in maintaining stem cell pluripotency by regulating the expression of genes critical to stem cell renewal and differentiation.² In other words, these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm). Nanog may be useful in the immunohistochemical diagnosis of tumors. It is expressed in germ cells of the fetus and in some germ cell tumors of the gonads³ and central nervous system.⁴

SVDPACPQSL PCFEASDCKE SSPMPVICGP EENYPSLQMS SAEMPHTETV SPLPSSMDLL IQDSPDSSTS PKGKQPTSAE NSVAKKEDKV PVKKQKTRTV FSSTQLCVLN DRFQRQKYLS LQQMQELSNI LNLSYKQVKT WFQNQRMKSK RWQKNNWPKN SNGVTQKASA PTYPSLYSSY HQGCLVNPTG NLPMWSNQTW NNSTWSNQTQ NIQSWSNHSW NTQTWCTQSW NNQAWNSPFY NCGEESLQSC MQFQPNSPAS DLEAALEAAG EGLNVIQQTT RYFSTPQTMD LFLNYSMNMQ PEDV

Sterile Filtered solution.

Recombinant Human NANOG produced in Escherichia coli is a single, non-glycosylated polypeptide chain consisting of 305 amino acids (residues 1–305) with a calculated molecular mass of approximately 34.6 kDa. The protein is purified to homogeneity using proprietary chromatographic techniques.

The protein contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 5% glycerol.

- Short-Term (2-4 weeks): Store the entire vial at 2-8°C.
- Long-Term: Freeze at -20°C.. To ensure stability during long-term storage, it is advisable to include a carrier protein (such as 0.1% Human Serum Albumin or Bovine Serum Albumin).
Avoid Repeated Freezing and Thawing Cycles.

1. Schöler HR.et al. (2003) Cell 113: 551 2. Pei D. et al. (2005) J Biol. Chem. 280: 1401 3. Rajpert-De Meyts E. et al. (2005) Histopathology 47: 48 4. Ligon KL. et al. (2006) Am J Surg Pathol. 30: 1613