The predicted molecular weight of Recombinant Mouse FGF R2β (IIIb) is Mr 56 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 74 kDa.
Predicted Molecular Mass
56
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
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Recombinant Mouse FGF R2β (IIIb) is a critical tool for research focused on mesenchymal-epithelial signaling, organogenesis, and tissue patterning in mouse models. This receptor isoform is the epithelial splice variant of fibroblast growth factor receptor 2 (FGFR2), and its biological importance is well-established in developmental biology and disease modeling.
Key reasons to use Recombinant Mouse FGF R2β (IIIb) in research applications:
Essential for Mesenchymal-Epithelial Signaling: FGFR2(IIIb) mediates signals from mesenchymal-derived FGFs to epithelial cells, a process fundamental to the development of multiple organs including limbs, lungs, pituitary gland, salivary glands, inner ear, teeth, skin, and skull.
Developmental Biology and Disease Modeling: Knockout studies in mice show that loss of FGFR2(IIIb) leads to severe developmental defects and extensive apoptosis in affected tissues, highlighting its indispensable role in early organogenesis.
Tissue-Specific Expression: The IIIb isoform is predominantly expressed in epithelial tissues, making it highly relevant for studies on epithelial-mesenchymal interactions, tissue regeneration, and epithelial-derived cancers.
Receptor-Ligand Interaction Studies: Recombinant FGFR2β (IIIb) enables in vitro assays to characterize ligand specificity, binding kinetics, and downstream signaling of FGF family members, particularly those that preferentially bind the IIIb isoform.
Cell Signaling and Functional Assays: The recombinant protein can be used in ELISA, cell-based assays, and biochemical studies to dissect FGF signaling pathways, receptor activation, and cellular responses such as proliferation, differentiation, and migration.
Protein Quality and Stability: The recombinant protein is typically produced with high purity (>90%), low endotoxin levels, and is stable under recommended storage conditions, ensuring reproducibility and reliability in experimental setups.
Applications include:
ELISA and ligand-binding assays to quantify or characterize FGF-FGFR2(IIIb) interactions.
Cell culture experiments to study epithelial cell responses to FGF signaling.
Developmental biology models to investigate the consequences of altered FGFR2(IIIb) signaling.
Screening of therapeutic candidates targeting FGF signaling in epithelial tissues.
In summary, Recombinant Mouse FGF R2β (IIIb) is indispensable for dissecting the molecular mechanisms of epithelial development, regeneration, and disease, especially where precise control and characterization of FGF signaling are required.
Recombinant Mouse FGF R2β (IIIb) is not suitable as a standard for quantification or calibration in ELISA assays designed to measure FGF-2 (FGF basic) or FGF-21. Here’s why:
1. Different Target Molecules
FGF R2β (IIIb) is a receptor for FGF ligands, not an FGF ligand itself.
ELISA kits for FGF-2 (bFGF) or FGF-21 are designed to detect the ligand (the growth factor), not its receptor.
Using a receptor as a standard in a ligand-specific ELISA will not generate a valid standard curve because the antibodies in the kit are specific for the ligand (FGF-2 or FGF-21), not the receptor.
2. Kit-Specific Standards
ELISA kits for mouse FGF-2 (e.g., R&D Systems Quantikine MFB00, DuoSet DY3139) and FGF-21 (e.g., R&D Systems Quantikine MF2100) are calibrated using recombinant mouse FGF-2 or recombinant mouse FGF-21, respectively.
These kits include lyophilized standards of the correct ligand, which are essential for accurate quantification.
3. Cross-Reactivity Data
Cross-reactivity studies (e.g., in R&D Systems DuoSet ELISA Development System datasheets) show that recombinant mouse FGF R2β (IIIb) does not cross-react with antibodies specific for FGF-2 or FGF-21.
This means the ELISA will not detect the receptor, and thus, it cannot be used to generate a standard curve.
4. Best Practice
For accurate quantification, always use the recombinant ligand (FGF-2 or FGF-21) as the standard, as provided in the ELISA kit or from a reputable supplier.
If you need to measure FGF R2β (IIIb) levels, use an ELISA kit specifically designed for that receptor.
Summary
No, you should not use recombinant Mouse FGF R2β (IIIb) as a standard for FGF-2 or FGF-21 ELISA assays. Use the appropriate recombinant ligand (FGF-2 or FGF-21) for calibration and quantification.
Based on the provided search results, Recombinant Mouse FGF R2β (IIIb) has been validated for the following applications in published research:
ELISA (Enzyme-Linked Immunosorbent Assay): The protein is specifically validated for use in ELISA to quantify FGF R2β (IIIb), provided that appropriate standards and antibodies targeting FGF R2β (IIIb) are used. This is highlighted in the product details from Leinco Technologies, which also notes that protocols and assay formats can be tailored for specific research needs.
Cell Culture Applications: The protein is suitable for use in cell culture systems, with protocols designed to ensure maximal stability and bioactivity. This is relevant for studies involving mesenchymal-epithelial signaling, organogenesis, and tissue patterning.
Research on Mesenchymal-Epithelial Signaling and Organogenesis: Published studies, such as the one referenced in PubMed (PMID: 10631169), demonstrate the biological relevance of FGF R2β (IIIb) in mesenchymal-epithelial signaling during mouse organogenesis. Knockout studies in mice show that loss of FGFR2(IIIb) leads to severe developmental defects in organs such as limbs, lungs, pituitary gland, salivary glands, inner ear, teeth, skin, and skull, underscoring its role in early organogenesis and tissue patterning.
These applications are supported by both product-specific documentation and peer-reviewed research, making Recombinant Mouse FGF R2β (IIIb) a valuable tool for studies in developmental biology, cell signaling, and tissue engineering.
To reconstitute and prepare Recombinant Mouse FGF R2β (IIIb) protein for cell culture experiments, follow these steps for optimal stability and bioactivity:
Centrifuge the vial briefly before opening to ensure all lyophilized protein is at the bottom.
Aseptically reconstitute the lyophilized protein in sterile buffer. The manufacturer’s datasheet specifies that the protein is lyophilized from modified Dulbecco’s phosphate buffered saline (PBS), pH 7.2–7.3, with no calcium, magnesium, or preservatives. Therefore, reconstitute using sterile PBS (pH 7.2–7.4) or sterile distilled water if PBS is not available.
Recommended concentration: While the datasheet does not specify an exact concentration for reconstitution, similar recombinant FGF receptor proteins are typically reconstituted at 0.1–1 mg/mL. Adjust the volume to achieve your desired working concentration, considering your downstream application.
Mix gently by pipetting up and down or gentle vortexing to fully dissolve the protein. Avoid vigorous agitation to prevent denaturation.
Aliquot the reconstituted protein to minimize freeze-thaw cycles, which can reduce activity.
Storage after reconstitution:
Store at 2–8°C for up to one month for short-term use.
For long-term storage, freeze aliquots at –20°C to –70°C in a manual defrost freezer.
Avoid repeated freeze-thaw cycles.
Carrier protein (optional): If the protein will be diluted to low concentrations (<0.1 mg/mL) or stored for extended periods, consider adding a carrier protein such as 0.1% BSA to prevent adsorption to tube walls and enhance stability.
Additional notes for cell culture use:
Ensure all solutions and handling are sterile to prevent contamination.
If using in functional assays, confirm the absence of endotoxin contamination (should be <1.0 EU/µg as per datasheet).
For quantification or detection, use validated ELISA kits specific for FGF R2β (IIIb) if needed.
Summary protocol:
Centrifuge vial → Add sterile PBS or water → Dissolve gently → Aliquot → Store at 2–8°C (short-term) or –20°C to –70°C (long-term) → Avoid freeze-thaw cycles.
Always consult the lot-specific datasheet for any additional or updated instructions.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
