Using Recombinant Mouse HGF R (Hepatocyte Growth Factor Receptor, c-Met) in research applications is essential for studying the biological mechanisms and therapeutic potential of HGF/c-Met signaling, which is involved in cell proliferation, motility, survival, tissue regeneration, and disease pathogenesis.

Key reasons to use Recombinant Mouse HGF R in your research:

• Modeling HGF/c-Met Signaling: Recombinant Mouse HGF R enables precise investigation of the HGF/c-Met pathway, which regulates epithelial morphogenesis, cell scattering, branching tubulogenesis, and integrin expression. This pathway is critical in development, regeneration, and cancer biology.

• Functional Assays and Bioactivity Studies: Recombinant proteins provide high bioactivity and batch-to-batch consistency, making them ideal for reproducible cell-based assays, organoid cultures, and in vivo studies. This is particularly important for dissecting the effects of HGF/c-Met interactions on cell behavior.

• Disease Modeling and Regenerative Medicine: The HGF/c-Met axis is implicated in liver regeneration, wound healing, muscle atrophy reversal, and protection against tissue injury. Recombinant HGF R can be used to model these processes in vitro and in vivo, and to test therapeutic interventions.

• Cancer and Fibrosis Research: Aberrant HGF/c-Met signaling is associated with tumor progression, metastasis, and tissue fibrosis. Recombinant Mouse HGF R allows for controlled studies on how modulating this pathway affects disease outcomes.

• Neuroscience and Immunology: HGF/c-Met signaling has neuroprotective, pro-angiogenic, anti-inflammatory, and immune-modulatory effects, making recombinant HGF R valuable for studies in neurological disorders and immune responses.

• Consistency and Reproducibility: Recombinant proteins, including Mouse HGF R, offer defined composition and purity, reducing experimental variability compared to tissue-derived proteins.

Typical applications include:

• Cell signaling and migration assays
• Organoid and stem cell culture systems
• In vivo models of tissue injury, regeneration, and disease
• Screening for therapeutic compounds targeting the HGF/c-Met pathway

In summary, Recombinant Mouse HGF R is a critical tool for mechanistic studies and translational research focused on growth factor signaling, tissue regeneration, cancer, and related fields, due to its biological relevance, reproducibility, and versatility in experimental systems.

Yes, recombinant Mouse HGF R (Hepatocyte Growth Factor Receptor) can be used as a standard for quantification or calibration in ELISA assays, provided the assay is designed to detect the recombinant form and the standard is properly formulated and validated for this purpose.

Essential context and supporting details:

• Recombinant proteins are commonly used as ELISA standards because they provide a defined concentration and purity, allowing for accurate calibration of the assay.
• ELISA kits for mouse HGF routinely use recombinant mouse HGF as the standard to generate the calibration curve, enabling quantification of HGF in biological samples.
• It is important to ensure that the recombinant protein matches the analyte detected by the ELISA antibodies (i.e., the same isoform, sequence, and post-translational modifications if relevant).
• The recombinant protein should be formulated appropriately (often with BSA for stability) and diluted in the same buffer as the samples to minimize matrix effects.
• Validation data from ELISA kit manufacturers show that recombinant mouse HGF produces dose-response curves parallel to those of natural HGF, supporting its use as a standard for quantification.

Best practices:

• Prepare a standard curve with serial dilutions of the recombinant Mouse HGF R in the same diluent as your samples.
• Confirm that your ELISA kit or custom assay recognizes both recombinant and native HGF with similar affinity and specificity.
• Validate the linearity and accuracy of quantification by spiking known amounts of recombinant HGF into sample matrices and comparing recovery rates.

Additional relevant information:

• If your assay is designed to detect the HGF receptor (c-MET) rather than HGF ligand, ensure the recombinant standard matches the target protein for your specific ELISA format.
• Always consult the technical documentation of your ELISA kit to confirm compatibility with recombinant standards and follow recommended protocols for standard preparation.

In summary, recombinant Mouse HGF R is suitable as a standard for ELISA quantification if it is matched to the assay target and validated for use in your specific protocol.

Applications of Recombinant Mouse HGF in Published Research

Recombinant mouse hepatocyte growth factor (HGF) has been validated across a diverse range of research applications, spanning from basic cell biology to regenerative medicine and disease modeling.

Cell-Based and Molecular Applications

The protein has been extensively used in bioassay applications to study HGF signaling mechanisms and cellular responses. Researchers employ recombinant mouse HGF in whole cell assays to investigate signal transduction pathways, particularly the HGF/c-Met axis, which regulates critical cellular processes including proliferation, migration, and morphogenesis.

Liver Regeneration and Disease Models

One of the most well-established applications involves studying liver regeneration and hepatoprotection. Recombinant HGF has been administered in mouse models to evaluate its therapeutic potential in alcoholic liver disease, where it successfully reduced liver injury markers (AST and ALT levels), decreased hepatic steatosis, enhanced antioxidant responses, and promoted hepatocyte proliferation. The protein has similarly been investigated in non-alcoholic fatty liver disease models, where it demonstrated anti-inflammatory, anti-fibrotic, and anti-lipogenic effects.

In Vivo Therapeutic Studies

Recombinant mouse HGF has been validated in in vivo applications through intravenous injection studies in disease models. These studies have demonstrated dose-dependent therapeutic effects, with researchers measuring outcomes through liver histology, biochemical indicators, and hepatocyte proliferation markers such as PCNA labeling.

Stem Cell and Tissue Engineering

The protein has been utilized to study stem cell biology and progenitor cell expansion. Research has shown that HGF promotes the expansion of hepatic stem/progenitor cells through ERK and STAT3-dependent signaling pathways, and it enhances the self-renewal capacity of embryonic stem cell-derived progenitors when combined with organ-matched mesenchyme.

Cancer and Metastasis Research

Recombinant mouse HGF has been applied in studies examining the HGF/c-Met pathway in cancer biology, including investigations of metastasis formation and malignant conversion in various tumor models.

Immunological Applications

The protein has been validated for studying immune tolerance and inflammation modulation, with mesenchymal stem cells shown to utilize HGF to alleviate acute lung injury through paracrine signaling mechanisms.

Analytical and Detection Methods

Recombinant mouse HGF serves as a standard in ELISA assays for measuring endogenous HGF levels in biological samples and for validating detection methodologies.

To reconstitute and prepare Recombinant Mouse HGF R (c-MET) protein for cell culture experiments, follow these best-practice steps:

1. Centrifuge the vial briefly before opening to ensure all lyophilized protein is at the bottom.

2. Reconstitution:

   • Add sterile distilled water or sterile PBS to the vial. Most protocols recommend reconstituting to a final concentration of at least 0.1 mg/mL (100 μg/mL).
   • If the product datasheet specifies, include 0.1% carrier protein (such as bovine or human serum albumin) in PBS to stabilize the protein and prevent adsorption to plastic surfaces.
   • Gently mix by swirling or inverting; avoid vigorous shaking or vortexing to prevent protein denaturation and foaming.
   • Allow the solution to sit at room temperature for 15–30 minutes to ensure complete dissolution.

3. Aliquoting and Storage:

   • Divide the reconstituted protein into small aliquots to avoid repeated freeze-thaw cycles, which can degrade protein activity.
   • Store aliquots at –20°C to –70°C for long-term storage. For short-term use (up to 1 month), store at 2–8°C under sterile conditions.
   • Avoid multiple freeze-thaw cycles.

4. Preparation for Cell Culture:

   • Before adding to cell culture, dilute the reconstituted stock to the desired working concentration using sterile cell culture medium or PBS.
   • Typical working concentrations for functional assays range from 1–100 ng/mL, but optimal concentrations should be determined empirically for your specific application.

Key technical notes:

• Always consult the specific product’s Certificate of Analysis or datasheet for any unique instructions, as buffer composition and recommended concentrations may vary by manufacturer and protein construct.
• If the protein is prone to aggregation or instability, the inclusion of a carrier protein (e.g., 0.1% BSA or HSA) is strongly recommended.
• For bioactivity assays, confirm the protein is fully dissolved and filter-sterilize if necessary using a 0.2 μm filter.

Summary protocol example:

1. Briefly centrifuge vial.2. Add sterile PBS (with 0.1% BSA if recommended) to achieve 100 μg/mL.3. Gently mix and let stand at room temperature for 15–30 min.4. Aliquot and store at –20°C to –70°C.5. Dilute to working concentration in cell culture medium before use.

This protocol ensures the recombinant Mouse HGF R protein is prepared in a manner that preserves its activity and suitability for cell culture experiments.