Recombinant Human HGF R

Hepatocyte growth factor receptor (HGFR), also known as c-Met, plays an important roles in angiogenesis and tumor growth.¹ C-Met is a receptor tyrosine kinase expressed by epithelial cells of the brain, kidney, liver and other tissues. Binding of its ligand, Hepatocyte Growth Factor (HGF), triggers receptor autophosphorylation, and activation of several downstream effectors including the mitogen-activated protein kinases ERK-1 and ERK-2, and PLC-γ. Activation of the c-Met signal transduction pathway leads to mulitple cellular responses including cell motility, scattering, proliferation, survival and angiogenesis.^2,3

The biological activity of Human HGF R was determined by its ability to bind human HGF. Immobilized rhHGF R/Fc at 1 μg/ml (100 μL/well) can bind rhHGF (0.1 - 5 ng/ml) in an ELISA type assay.

Fc Fusion Protein

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Glu25 & Ser308

Lyophilized

This protein consists of two disulfide-linked heterodimers (proteolytically cleaved α and β subunits). The predicted molecular mass is 32.5 kDa (α chain) and 96.7 kDa (β chain). The actual molecular mass of Recombinant Human HGF R is Mr 43-50 kDa (α chain) and 120-125 kDa (β chain).

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) with trehalose pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. McDonald, DM. et al. (2008) BMB Rep. 41: 833
2. Park, M. et al. (1998) Oncogene 16: 833
3. Comoglio, PM. et al. (1998) J Cell Sci. 111: 237