Recombinant Mouse IL-22

Recombinant Mouse IL-22 is widely used in research applications to study its unique roles in tissue protection, regeneration, mucosal immunity, and inflammation, particularly in murine models. Its use enables precise control over cytokine levels and timing, facilitating mechanistic studies and therapeutic modeling.

Key scientific reasons to use recombinant Mouse IL-22 include:

• Tissue Protection and Regeneration: IL-22 promotes epithelial cell survival, proliferation, and repair, making it valuable for investigating tissue-protective mechanisms in models of liver, lung, pancreas, intestine, and skin injury. It activates the STAT3 pathway, leading to anti-apoptotic and pro-regenerative effects in epithelial tissues.

• Modeling Inflammatory and Infectious Diseases: IL-22 is critical for host defense against extracellular pathogens and regulates genes involved in innate immunity, such as antimicrobial peptides and acute phase reactants. Recombinant IL-22 allows researchers to dissect its role in infection, inflammation, and wound healing, especially at mucosal surfaces.

• Studying Immune-Epithelial Crosstalk: IL-22 is produced by immune cells (e.g., Th17 cells, dendritic cells) but acts primarily on non-immune, epithelial cells, making it a key mediator of immune-epithelial communication. Recombinant protein enables controlled studies of these interactions.

• Therapeutic Modeling: Preclinical studies support the use of recombinant IL-22 for testing tissue-protective therapies in acute epithelial injuries, with applications in models of pancreatitis, hepatitis, colitis, and cutaneous wounds. Its administration can break cycles of cell death-driven inflammation and promote healing.

• Mechanistic Studies: Recombinant IL-22 is essential for experiments requiring defined cytokine concentrations, timing, and delivery routes (e.g., intraperitoneal injection), allowing for reproducible mechanistic investigations.

• Mucosal Immunity and Autoimmunity: IL-22 plays a critical role in mucosal immunity and is implicated in dysregulated inflammation in autoimmune diseases, making it a target for studies on immune regulation and pathology.

Best practices for using recombinant Mouse IL-22:

• Employ in dose-response and time-course studies to determine optimal concentrations and effects.
• Use in combination with genetic models (e.g., knockout mice) to dissect endogenous versus exogenous IL-22 functions.
• Validate biological activity in relevant cell types (e.g., epithelial cells) via downstream signaling (STAT3 phosphorylation) and functional assays (e.g., induction of antimicrobial peptides).

Limitations and considerations:

• Prolonged or excessive IL-22 administration may have tumorigenic potential due to sustained STAT3 activation.
• Timing of administration is critical; IL-22 promotes proliferation, which may be undesirable before clearance of damaged cells.
• Context-specific effects: IL-22 can amplify certain aspects of autoimmune inflammation depending on disease model and timing.

In summary, recombinant Mouse IL-22 is a powerful tool for elucidating cytokine-mediated tissue protection, regeneration, and immune regulation in murine models, with broad applications in inflammation, infection, and therapeutic research.

Yes, recombinant mouse IL-22 can be used as a standard for quantification or calibration in ELISA assays, provided it is of high purity and its concentration is accurately known. This is a common practice in cytokine ELISAs, including those for IL-22.

Supporting details:

• ELISA kits for mouse IL-22 routinely use recombinant mouse IL-22 as the standard to generate calibration curves for quantification of IL-22 in samples. The standard curve is typically constructed using serial dilutions of recombinant IL-22 of known concentration.
• Assay validation data show that recombinant and natural mouse IL-22 produce parallel standard curves, indicating that recombinant IL-22 is suitable for accurate quantification of endogenous IL-22 in biological samples.
• Kit instructions and technical datasheets specify that both natural and recombinant mouse IL-22 can be quantified using these assays, and recombinant IL-22 is often provided as the standard in commercial kits.
• Best practices: Ensure the recombinant IL-22 is well-characterized (purity, activity, and concentration verified), and that it is reconstituted and diluted according to the ELISA protocol. Use the same buffer system as recommended for the assay to avoid matrix effects.

Additional considerations:

• If you are developing your own ELISA or using a kit without a provided standard, confirm that your recombinant IL-22 matches the sequence and post-translational modifications (if any) expected by the assay antibodies.
• Always run a standard curve in parallel with your samples for each assay plate to ensure accurate quantification.

In summary: Recombinant mouse IL-22 is widely accepted and validated as a standard for ELISA quantification of IL-22, as long as it is properly prepared and handled.

Recombinant Mouse IL-22 has been validated in published research for several key applications, primarily in preclinical models of epithelial injury, tissue protection, metabolic syndrome, and inflammatory disease.

Validated Applications in Published Research:

• Tissue Protection and Regeneration: Recombinant mouse IL-22 has been used to promote tissue protection and regeneration in murine models of liver, pancreatic, intestinal, and lung pathophysiology, particularly where epithelial injury is present. These studies highlight its role in activating the IL-22/STAT3 axis for tissue repair and modulation of inflammation.

• Metabolic Syndrome and Liver Disease: IL-22 has been validated for reducing markers of metabolic syndrome, including nonalcoholic and alcoholic fatty liver disease, in mouse models. Administration of recombinant IL-22 (either directly or via engineered probiotic delivery) resulted in decreased liver weight, reduced triglycerides, and amelioration of steatohepatitis.

• Regulation of Neutrophil Recruitment and Colitis: IL-22 has been shown to regulate neutrophil recruitment in the colon, impacting the expression of neutrophil-active chemokines and playing a role in models of ulcerative colitis and other inflammatory bowel diseases.

• Antimicrobial Immunity and Barrier Function: Recombinant mouse IL-22 has been used to study its effects on antimicrobial immunity and tissue repair at barrier surfaces, such as the gut and skin, by promoting the expression of antimicrobial peptides and supporting epithelial integrity.

• Cell Culture and ELISA Standard: Recombinant mouse IL-22 is commonly used as a standard in ELISA assays and for stimulation in cell and tissue culture experiments to investigate cytokine signaling and downstream effects.

Supporting Details:

• In liver disease models, IL-22 administration (intravenous or via engineered Lactobacillus reuteri) has been shown to reduce liver injury and inflammation, control lipid metabolism, and activate STAT3 signaling.
• In colitis and epithelial injury models, IL-22 promotes tissue repair, regulates immune cell recruitment, and enhances antimicrobial peptide production.
• Recombinant IL-22 is also used in mechanistic studies to dissect cytokine signaling pathways, especially those involving STAT3 activation and epithelial cell responses.

Summary Table of Validated Applications

These applications are supported by multiple peer-reviewed studies and reviews, demonstrating the utility of recombinant mouse IL-22 in both basic and translational research contexts.

To reconstitute and prepare Recombinant Mouse IL-22 protein for cell culture experiments, follow these best-practice steps:

• Centrifuge the vial briefly before opening to ensure all lyophilized powder is at the bottom.
• Reconstitute the lyophilized protein in sterile water or sterile PBS. The recommended concentration for stock solutions is typically 0.1–1.0 mg/mL (100–1000 μg/mL). For example, add 100 μL of sterile water to 100 μg of protein for a 1 mg/mL solution.
• Gently mix by swirling or inverting the vial. Do not vortex or shake vigorously, as this can impair biological activity.
• Incubate the solution at room temperature for at least 20 minutes to ensure complete dissolution.
• If the product datasheet specifies, use PBS with 0.1% BSA or another carrier protein to stabilize the protein, especially for long-term storage or repeated freeze-thaw cycles.
• Aliquot the reconstituted protein to avoid repeated freeze-thaw cycles. Store aliquots at –20°C to –80°C for long-term storage, or at 4°C for short-term use (up to 1 month).
• For cell culture, dilute the stock solution into your culture medium to the desired working concentration immediately before use.

Additional notes:

• Always consult the specific product datasheet for any manufacturer-specific recommendations regarding buffer composition, concentration, or additives.
• If the protein is formulated with stabilizers (e.g., trehalose, mannitol, Tween 80), these are generally compatible with cell culture but verify if your application is sensitive to these excipients.
• Avoid repeated freeze-thaw cycles, as this can reduce protein activity.

Summary protocol:

1. Briefly centrifuge vial.
2. Add sterile water or PBS to achieve 0.1–1.0 mg/mL.
3. Gently mix, do not vortex.
4. Incubate at room temperature for 20 min.
5. Aliquot and store at –20°C to –80°C.
6. Dilute into cell culture medium as needed.

These steps will ensure optimal solubility and activity of recombinant mouse IL-22 for cell culture experiments.